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Updated: Sep 9, 2025

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
Novel compound bacterial agent effects on pig farm wastewater denitrification and nitrogen transformation
Rong Gao1, Zhuangzhuang Liu1, Weikun Li1
1College of Bioscience and Biotechnology, Hunan Agricultural University, Hunan Engineering Laboratory for Pollution Control and Waste Utilization in Swine Production, Changsha, Hunan 410128, China.
Abstract:
This research involved the screening and combination of Escherichia coli, Acinetobacter radioresistens, and Klebsiella michiganensis to create a novel compound bacterial agent known as SSF-1. The efficacy of SSF-1 in treating real pig farm wastewater was assessed, focusing on its impact on nitrogen transformation, the structure of bacterial communities, and functional genes. SSF-1 demonstrated stable ammonia nitrogen conversion under both weakly acidic and weakly alkaline conditions, exhibiting superior environmental adaptability compared to individual strains. Under optimal conditions, the ammonia nitrogen conversion rate in simulated wastewater reached 98.4 ± 0.2 %. Furthermore, SSF-1 was found to synergistically treat wastewater with functional bacteria in the sludge. Metagenomic analysis revealed that SSF-1 enhanced nitrogen transformation and the reduction of nitrate/nitrite by upregulating key nitrogen metabolism genes. This discovery expands the application potential of compound bacterial agent in actual environments and contributes to the sustainable utilization of water resources.
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