Related Experiment Video
Updated: Sep 9, 2025
09:51
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
Published on: May 25, 2018
34.4K
A CRISPR Cas protein coronated AuNP nanostructure for enhanced uptake efficiency into cells.
Zhaojia Deng1,2, Rui Sha1, Hua Qin1,3
1State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. hypeng@rcees.ac.cn.
Chemical Communications (Cambridge, England)
|September 2, 2025
Summary
Researchers created a stable nanostructure using gold nanoparticles (AuNPs) and CRISPR Cas proteins. This innovation improves cellular uptake and stability for better imaging and biomedical uses.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Molecular Biology
Background:
- Nanoparticle effectiveness is hindered by aggregation in biological settings.
- Developing stable and efficiently uptaken nanostructures is crucial for biomedical applications.
Purpose of the Study:
- To engineer a protein-coronated nanostructure for enhanced stability and cellular uptake.
- To overcome the limitations of non-specific aggregation in nanotechnologies.
Main Methods:
- Functionalization of gold nanoparticles (AuNPs) with nucleic acid scaffolds.
- Incorporation of CRISPR Cas proteins to form a protein coronated nanostructure.
Main Results:
- The developed nanostructure demonstrated significantly enhanced nanoparticle stability.
- Cellular uptake efficiency was markedly improved compared to conventional nanoparticles.
- The nanostructure showed potential for advanced imaging and therapeutic delivery.
Conclusions:
- Protein-coronated nanostructures offer a viable strategy to improve nanoparticle performance in biological environments.
- This approach enhances stability and cellular uptake, paving the way for novel biomedical applications.
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