Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nuclear Overhauser Enhancement (NOE)01:07

Nuclear Overhauser Enhancement (NOE)

826
Irradiation of a spin-active nucleus causes an increase or decrease in the signal intensity of neighboring nuclei that are not necessarily chemically bonded or involved in J-coupling.  This phenomenon, called the Nuclear Overhauser Enhancement (NOE), results from through-space interactions between the nuclear spins. The NOE effect decreases with increasing internuclear distance and is generally not observed beyond 4 angstroms. In NOE, dipole-dipole interactions between neighboring...
826
Atomic Nuclei: Nuclear Relaxation Processes01:23

Atomic Nuclei: Nuclear Relaxation Processes

718
In the absence of an external magnetic field, nuclear spin states are degenerate and randomly oriented. When a magnetic field is applied, the spins begin to precess and orient themselves along (lower energy) or against (higher energy) the direction of the field. At equilibrium, a slight excess population of spins exists in the lower energy state. Because the direction of the magnetic field is fixed as the z-axis,  the precessing magnetic moments are randomly oriented around the z-axis.
718
Atomic Nuclei: Nuclear Spin State Population Distribution01:14

Atomic Nuclei: Nuclear Spin State Population Distribution

1.2K
Near absolute zero temperatures, in the presence of a magnetic field, the majority of nuclei prefer the lower energy spin-up state to the higher energy spin-down state. As temperatures increase, the energy from thermal collisions distributes the spins more equally between the two states. The Boltzmann distribution equation gives the ratio of the number of spins predicted in the spin −½ (N−) and spin +½ (N+) states.
1.2K
Atomic Nuclei: Nuclear Spin State Overview01:03

Atomic Nuclei: Nuclear Spin State Overview

1.1K
NMR-active nuclei have energy levels called 'spin states' that are associated with the orientations of their nuclear magnetic moments. In the absence of a magnetic field, the nuclear magnetic moments are randomly oriented, and the spin states are degenerate. When an external magnetic field is applied, the spin states have only 2 + 1 orientations available to them. A proton with = ½ has two available orientations. Similarly, for a quadrupolar nucleus with a nuclear spin value of...
1.1K
Atomic Nuclei: Nuclear Magnetic Moment00:59

Atomic Nuclei: Nuclear Magnetic Moment

1.4K
All atomic nuclei are positively charged. When they have a nonzero spin, they behave like rotating charges. As a consequence of their charge and spin, these nuclei generate a magnetic field (B). This, in turn, gives rise to a magnetic moment (μ), which is randomly oriented in the absence of an external magnetic field. When an external magnetic field (B0) is applied, the magnetic moment vectors can align with the field or against it in 2 + 1 orientations. A hydrogen nucleus, which is just a...
1.4K
Nuclear Binding Energy02:13

Nuclear Binding Energy

13.0K
The difference between the calculated and experimentally measured masses is known as the mass defect of the atom. In the case of helium-4, the mass defect indicates a “loss” in mass of 4.0331 amu – 4.0026 amu = 0.0305 amu. The loss in mass accompanying the formation of an atom from protons, neutrons, and electrons is due to the conversion of that mass into energy that is evolved as the atom forms. The nuclear binding energy is the energy produced when the atoms’ nucleons...
13.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhancing <sup>29</sup>Si Hyperpolarization Efficiency in Silica Nanoparticles <i>via</i> Multishell Design with Selective <sup>29</sup>Si-Isotope and Radical Enrichment.

Analytical chemistry·2025
Same author

Dual-Emissive Mn-Doped Lead Halide Perovskite Nanocrystals as Background-Suppressed Latent Fingerprint Detection Probes.

ACS applied materials & interfaces·2023
Same author

Chemical Analysis of an Isotopically Labeled Molecule Using Two-Dimensional NMR Spectroscopy at 34 μT.

ACS omega·2023
Same author

Frequency Limits of Sequential Readout for Sensing AC Magnetic Fields Using Nitrogen-Vacancy Centers in Diamond.

Sensors (Basel, Switzerland)·2023
Same author

Anti-obesity effects of Camellia (Camellia oleifera Abel) oil treatment on high-fat diet-induced obesity in C57BL/6J mice.

Physical activity and nutrition·2023
Same author

Wettability of polytetrafluoroethylene surfaces by plasma etching modifications.

PloS one·2023
Same journal

NMR Spectroscopy: Molecular Insights into Cell Wall Collapse and Oxidative Stress of <i>Escherichia coli</i> Induced by Imidazole-Activated Eutectic Solvents.

ACS omega·2026
Same journal

Enhanced Arsenite Remediation in Synthetic FeS<sub>2</sub>/Fe(II)-Containing Arsenic Wastewater via Epigallocatechin Gallate-Initiated Persulfate Activation.

ACS omega·2026
Same journal

Defect and Particle-Size Engineering as Mechanistic Drivers for Dye Uptake in a Zirconium Metal-Organic Framework.

ACS omega·2026
Same journal

Biogeochemical Assessment of Short-Term Hydrogen Storage in Methane Reservoirs with Field Sample Characterization and Reactor Experiments.

ACS omega·2026
Same journal

Combined Effects of Halloysite Nanotubes, Nucleating Agent, and Thermal Annealing on the Printability and Mechanical Performances of 3D-Printable Polypropylene Random Copolymer-Based Composites.

ACS omega·2026
Same journal

Effect of MoS<sub>2</sub> Interfacial Engineering across MAPbI<sub>3</sub>, FAPbI<sub>3</sub>, and CsPbI<sub>3</sub> Perovskite Solar Cells.

ACS omega·2026
See all related articles

Related Experiment Video

Updated: Sep 9, 2025

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
11:43

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

Published on: December 30, 2016

10.6K

Zero-Field Overhauser Dynamic Nuclear Polarization.

Seong-Joo Lee1, Kwon Kyu Yu2, Seong-Min Hwang1

  • 1Quantum Magnetic Sensing Group, Korea Research Institute of Standards and Science, Daejeon 34113, Republic of Korea.

ACS Omega
|September 2, 2025
PubMed
Summary
This summary is machine-generated.

This study demonstrates hyperpolarizing water using Overhauser dynamic nuclear polarization (O-DNP) at near-zero magnetic fields. This novel method significantly enhances O-DNP efficiency using circularly polarized radiofrequency fields.

More Related Videos

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
10:54

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Published on: February 23, 2016

10.8K
Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR
09:37

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

Published on: February 12, 2019

7.6K

Related Experiment Videos

Last Updated: Sep 9, 2025

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
11:43

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

Published on: December 30, 2016

10.6K
Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
10:54

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Published on: February 23, 2016

10.8K
Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR
09:37

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

Published on: February 12, 2019

7.6K

Area of Science:

  • Magnetic Resonance
  • Physical Chemistry
  • Biophysics

Background:

  • Overhauser dynamic nuclear polarization (O-DNP) typically requires significant magnetic fields for hyperpolarization.
  • Nitroxide radicals are commonly used to induce hyperpolarization in liquids.

Purpose of the Study:

  • To demonstrate experimental hyperpolarization of water using O-DNP at effectively zero magnetic field.
  • To investigate the efficiency of circularly polarized radiofrequency (RF) fields in O-DNP at low fields.

Main Methods:

  • Utilized circularly polarized RF fields generated by orthogonal RF coils to induce selective saturation.
  • Employed electron-to-nuclear spectral mapping to record the electron paramagnetic resonance spectrum at 2 nT.
  • Compared the efficiency of O-DNP using circularly polarized RF versus linearly polarized RF.

Main Results:

  • Achieved hyperpolarization of water via O-DNP at near-zero magnetic field.
  • Circumvented the detrimental effects of concurrent positive and negative polarizations using selective saturation.
  • Observed a >103-fold increase in O-DNP efficiency with circularly polarized RF compared to linearly polarized RF.
  • Recorded the nitroxide radical's EPR spectrum at a magnetic field as low as 2 nT.

Conclusions:

  • Water can be effectively hyperpolarized using O-DNP at near-zero magnetic fields.
  • Circularly polarized RF fields significantly enhance O-DNP efficiency at low fields.
  • Eliminated the need for auxiliary techniques like sample shuttling or field cycling for low-field O-DNP.