Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Noncovalent Attractions in Biomolecules02:35

Noncovalent Attractions in Biomolecules

54.5K
Noncovalent attractions are associations within and between molecules that influence the shape and structural stability of complexes. These interactions differ from covalent bonding in that they do not involve sharing of electrons.
Four types of noncovalent interactions are hydrogen bonds, van der Waals forces, ionic bonds, and hydrophobic interactions.
Hydrogen bonding results from the electrostatic attraction of a hydrogen atom covalently bonded to a strong-electronegative atom like oxygen,...
54.5K
Condensins02:15

Condensins

3.6K
Condensins are large protein complexes that use ATP to fuel the assembly of chromosomes during mitosis. They transform the tangled, shapeless mass of post-interphase DNA into individualized chromosomes by compacting, organizing, and segregating chromosomal DNA.
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...
3.6K
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.1K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.1K
¹H NMR of Conformationally Flexible Molecules: Temporal Resolution00:52

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

915
At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
915
Ketones with Nonenolizable Aromatic Aldehydes: Claisen–Schmidt Condensation01:01

Ketones with Nonenolizable Aromatic Aldehydes: Claisen–Schmidt Condensation

3.8K
Benzaldehyde, like formaldehyde, lacks an α hydrogen and cannot enolize to form an enolate. Hence, the reaction of benzaldehyde with a ketone in the presence of an aqueous base forms a single crossed product. This reaction is referred to as Claisen–Schmidt condensation.
As the self-condensation of ketones is generally not favored in basic conditions, the self-condensed products do not form in the reaction between ketones and benzaldehyde. The general reaction of Claisen–Schmidt...
3.8K
Protein Folding01:22

Protein Folding

120.8K
Overview
120.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Crowder-Induced Conformational Fluctuations Modulate the Phase Separation of the Yeast Sup35NM Domain.

Biomacromolecules·2026
Same author

LncRNA YIYA drives pancreatic cancer proliferation under high-glucose conditions by reinforcing a RAS-PKM2-dependent Warburg phenotype.

FEBS letters·2026
Same author

A series of spontaneously blinking dyes for super-resolution microscopy.

Nature methods·2026
Same author

Cisplatin-Induced Oxidative Stress Regulates YAP to Modulate Epigenome Promoting the Survival of Osteosarcoma Cells.

Biochemistry·2026
Same author

Editor's Note: The Genetic Landscape of Ocular Adnexa MALT Lymphoma Reveals Frequent Aberrations in NFAT and MEF2B Signaling Pathways.

Cancer research communications·2026
Same author

A repeat expansion in GOLGA8A is a major risk factor for atypical frontotemporal lobar degeneration with ubiquitin-positive inclusions.

Nature genetics·2026
Same journal

A human-specific genetic modifier reconfigures large-scale cortical network dynamics underlying behavioral performance.

bioRxiv : the preprint server for biology·2026
Same journal

<i>Staphylococcus aureus</i> uses a eukaryotic-like uridyltransferase to make UDP-GlcNAc for cell wall synthesis.

bioRxiv : the preprint server for biology·2026
Same journal

Dynamic redistribution of eIF4F controls cap-dependent translation initiation.

bioRxiv : the preprint server for biology·2026
Same journal

When does additional information improve accuracy of RNA secondary structure prediction?

bioRxiv : the preprint server for biology·2026
Same journal

Normative brain-state trajectories reveal deviation from healthy aging in Alzheimer's disease.

bioRxiv : the preprint server for biology·2026
Same journal

Noradrenergic infraslow rhythm during sleep is the critical link between heart-rate dynamics and memory consolidation.

bioRxiv : the preprint server for biology·2026
See all related articles

Related Experiment Video

Updated: Sep 9, 2025

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells
06:48

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

Published on: January 5, 2024

4.1K

Clustering within a single-component biomolecular condensate.

Ankith Sharma1,2, Abhishek Sau1,2, Sandeep Dave1

  • 1Department of Cell Biology and Genetics, Texas A&M University; College Station, TX, USA.

Biorxiv : the Preprint Server for Biology
|September 2, 2025
PubMed
Summary
This summary is machine-generated.

Biomolecular condensates (BMCs) exhibit complex internal structures. Even simple Fused in Sarcoma (FUS) condensates show clustering and altered mobility over time, suggesting a sponge-like organization rather than a solid network.

More Related Videos

Spatial Separation of Molecular Conformers and Clusters
10:37

Spatial Separation of Molecular Conformers and Clusters

Published on: January 9, 2014

9.1K
Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures
08:02

Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures

Published on: May 31, 2024

900

Related Experiment Videos

Last Updated: Sep 9, 2025

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells
06:48

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

Published on: January 5, 2024

4.1K
Spatial Separation of Molecular Conformers and Clusters
10:37

Spatial Separation of Molecular Conformers and Clusters

Published on: January 9, 2014

9.1K
Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures
08:02

Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures

Published on: May 31, 2024

900

Area of Science:

  • Cell biology
  • Biophysics
  • Biochemistry

Background:

  • Biomolecular condensates (BMCs) are non-membrane-bound cellular compartments crucial for biological processes.
  • Their function depends on internal organization and molecular dynamics.
  • Altered BMCs are implicated in protein aggregation diseases.

Purpose of the Study:

  • To investigate the nanoscale structural and functional properties of Fused in Sarcoma (FUS) condensates.
  • To understand the dynamics of FUS monomers within BMCs over time.
  • To explore the relationship between condensate aging, structural changes, and molecular mobility.

Main Methods:

  • Single molecule microscopy
  • Advanced microscopy techniques
  • Analysis of condensate physical properties and monomer diffusion

Main Results:

  • Single-component FUS condensates show internal clustering and higher FUS density.
  • Aging leads to altered physical properties and reduced overall monomer mobility.
  • A significant fraction of FUS monomers maintain high mobility, indicating a sponge-like structure.
  • Condensates exhibit a pseudo-equilibrium between different structural connectivities.

Conclusions:

  • Simple FUS condensates possess nanoscale structural complexity.
  • The observed mobility patterns challenge the notion of a simple, system-spanning network.
  • These findings suggest that BMCs can harbor dynamic pseudo-equilibrium states, impacting their function.