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Updated: Sep 9, 2025

Matrix-based DNA Extraction for Targeted Next-Generation Sequencing on Decontaminated Sputum Samples
Published on: June 6, 2025
Comprehensive genomic insights into Mixta calida isolated from the faecal sample of a tuberculosis patient in India
Archana Pant1, Jayalakshmi Srinivasan1, Sureshkumar Venkadesan2
1Molecular Genetics Lab, National Institute of Immunology, New Delhi, India.
Abstract:
Mixta calida, formerly known as Pantoea calida, is a motile Gram-negative, facultatively anaerobic bacterium with coccoid rod morphology. Although previously considered non-pathogenic, emerging case studies indicate its potential role in causing serious infections, including bacteraemia, meningitis, sepsis, and implant-associated infections. This study presents the first whole-genome sequence of M. calida of Indian origin, isolated from the stool sample of a tuberculosis patient undergoing treatment. Sequencing was performed using the Illumina NextSeq 2000 and Oxford Nanopore PromethION platforms. The genomic data provides valuable insights into the antimicrobial resistance traits and mobile genetic elements of the bacterium, contributing to a deeper understanding of its pathogenic potential.
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