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Updated: Sep 9, 2025

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
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Published on: May 23, 2025

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CRISPR/Cas9 in colorectal cancer: Revolutionizing precision oncology through genome editing and targeted therapeutics

Bahjat Alhasso1, Abdulkareem Shareef2, Lalji Baldaniya3

  • 1College of Pharmacy, Alnoor University, Nineveh, Iraq.

Iranian Journal of Basic Medical Sciences
|September 2, 2025
PubMed

Insights

CRISPR/Cas9 gene editing offers new ways to fight colorectal cancer (CRC) by precisely altering genes in cancer cells. This technology shows promise in preclinical models for suppressing tumors and improving treatment responses.

Area of Science:

  • Genetics and Genomics
  • Oncology
  • Molecular Biology

Background:

  • Colorectal cancer (CRC) is a major global health concern requiring innovative molecular therapies.
  • The CRISPR/Cas9 system provides a powerful platform for precise genome editing in cancer research.

Purpose of the Study:

  • To review the applications of CRISPR/Cas9 technology in colorectal cancer research models.
  • To highlight the potential of CRISPR/Cas9 in suppressing tumor progression and enhancing therapeutic sensitivity.
  • To discuss the challenges and future directions for clinical translation of CRISPR/Cas9 in CRC.

Main Methods:

  • Utilized CRISPR/Cas9 in CRC models (MC38, CaCO-2) for targeted gene modifications.
  • Investigated the effects of gene knockouts (e.g., Par3L) on cancer cell proliferation, apoptosis, and chemosensitivity.
  • Explored adeno-associated virus (AAV)-mediated CRISPR editing in specific CRC models.

Main Results:

  • CRISPR/Cas9 gene editing demonstrated tumor-suppressive effects in preclinical CRC models.
  • Par3L protein knockout inhibited proliferation, induced apoptosis, and sensitized cells to chemotherapy via AMPK signaling.
  • AAV-mediated CRISPR editing showed potential in HPV16-driven CRC models.

Conclusions:

  • CRISPR/Cas9 is a transformative tool for CRC research, enabling precise genetic manipulation.
  • Overcoming challenges like off-target effects and delivery is crucial for clinical translation.
  • CRISPR/Cas9 holds significant promise for advancing precision oncology and personalized CRC therapeutics.
Keywords:
CRISPR/Cas9Colorectal Cancer (CRC) Gene TherapyGenome EditingOncogenic PathwaysTumor Suppression

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