Related Experiment Video
Updated: Sep 9, 2025
12:20
Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
Published on: December 29, 2015
21.5K
Transferrin receptor 1 is an entry factor for Japanese encephalitis virus
Qing Yang1, Shengda Xie1, Yundi Zhao1
1MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Veterinary Microbiology
|September 2, 2025
Summary
Japanese encephalitis virus (JEV) uses transferrin receptor 1 (TfR1) to enter cells. Inhibiting TfR1 with iron supplements like ferric ammonium citrate (FAC) offers a potential antiviral strategy against JEV infection.
Area of Science:
- Virology
- Cell Biology
- Infectious Diseases
Background:
- Japanese encephalitis virus (JEV) causes viral encephalitis in humans and animals.
- Understanding JEV entry mechanisms is crucial for developing antiviral therapies.
Purpose of the Study:
- To investigate the role of transferrin receptor 1 (TfR1) in JEV infection.
- To identify TfR1 as a potential antiviral target for JEV.
Main Methods:
- Knockdown and overexpression of TfR1 in A549, HeLa, and 293T cells.
- Investigated the interaction between JEV E protein and TfR1.
- Utilized soluble hTfR1 ectodomain protein and ferric ammonium citrate (FAC) to block JEV infection.
Main Results:
- TfR1 knockdown inhibited JEV entry, while overexpression enhanced it.
- The JEV E protein specifically interacts with TfR1 residues 384-606.
- Soluble TfR1 blocked JEV infection, and FAC downregulated TfR1 expression, inhibiting JEV entry and infection.
Conclusions:
- TfR1 is identified as a critical entry factor for JEV.
- TfR1 represents a promising antiviral target for JEV.
- Small molecule inhibitors like FAC show potential as an antiviral strategy against JEV.
Related Concept Videos
01:28The Early Endosome: Endocytosis of Transferrin
3.4K
Essential proteins such as insulin or low-density lipoprotein (LDL) and micronutrients such as iron enter a eukaryotic cell through receptor-mediated endocytosis. Subsequently, the early endosomes fuse with the vesicles containing such receptor-ligand complexes and play a vital role in sorting the incoming ligands and receptors. While the ligands are either degraded inside the vesicle or released into the cytosol, their receptors are returned to the plasma membrane for further rounds of...
3.4K
02:28Leaky Scanning
5.2K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.2K
01:27Transducer Mechanism: Enzyme-Linked Receptors
2.8K
Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
Major types that are helpful drug targets include:
2.8K
01:20Receptor-mediated Endocytosis
6.3K
Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
6.3K
01:00Enzyme-linked Receptors
79.9K
Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
79.9K