Related Experiment Videos
Observations on the number of available C antigen sites on red cells
Vox Sanguinis
|September 1, 1977
Insights
This study quantifies Rh C (rh
Area of Science:
- Immunology
- Genetics
- Hematology
Background:
- The Rh blood group system is crucial for transfusion medicine.
- Understanding antigen site density is vital for serological studies.
Purpose of the Study:
- To estimate the number of available C (rh', Rh 2) antigen sites on red blood cells.
- To determine the average equilibrium constant for anti-C antibodies.
Main Methods:
- Utilized trace-labelled antibodies for precise quantification.
- Employed quantitative serological techniques to measure antigen site density.
Main Results:
- Reported variable C antigen site numbers across different Rh genotypes (e.g., CDe/CDe: 45,700-56,400 sites/cell).
- Determined an average equilibrium constant of 0.5 x 10^7 M^-1 for anti-C.
Conclusions:
- Provides quantitative data on C antigen expression in various Rh phenotypes.
- Establishes a basis for further research into Rh antigen-antibody interactions.
Abstract:
The number of available C (rh', Rh 2) antigen sites within the Rh system was estimated using trace-labelled antibody. The results, as available sites per red cell, were as follows: CDe/CDe: 45,700-56,400; Cde/Cde: 42,200; CDe/cDE: 25,500-39,700; CwDe/cde: 21,500-40, 000; Cde/cde: 31,100; CDE/cDE: 8,500-9,800; Cde/cde: 7,200. The average equilibrium constant of the anti-C was 0.5 X 10(7) M-1.