Development of paper-based electrochemiluminescence test strips for pathogenic microorganisms.

Area of Science:

• Biosensors
• Analytical Chemistry
• Immunotechnology

Background:

• Development of a novel paper-based electrochemiluminescence test strip (ECL-TS).
• Integration of lateral flow immunochromatography (LFIC) with electrochemiluminescence (ECL) for enhanced detection.
• Focus on detecting pathogenic microorganisms.

Purpose of the Study:

• To create a portable, rapid, and sensitive detection method for pathogenic microorganisms.
• To evaluate the performance of the developed ECL-TS.
• To compare the method with existing detection techniques like nucleic acid testing.

Main Methods:

• Utilized a double-antibody sandwich principle with labeled and capture monoclonal antibodies.
• Employed LFIC for antibody flow and binding in the detection region.
• Applied cyclic voltammetry for ECL signal generation and quantification.

Main Results:

• Achieved detection within 5 minutes with a low limit of detection (7.96 pg/mL).
• Demonstrated good reproducibility (5.62% deviation) and stability (6.34% deviation).
• Exhibited high specificity for target viruses with a KAPPA value of 0.88.

Conclusions:

• The paper-based ECL-TS is a viable portable, rapid, and sensitive diagnostic tool.
• Demonstrated advantages in speed, accuracy, and cost-effectiveness compared to nucleic acid methods, using SARS-CoV-2 as a model.
• Potential application as a point-of-care testing (POCT) tool for various pathogenic microorganisms.