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An open source for multiplexed, stable and transient flows to advance life sciences using microfluidic control

Junichi Murai1,2, Mahmoud N Abdelmoez1,3, Keisuke Kondo1,2

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We developed microfluidic sequence automation (MiSA), an affordable, open-source system for precise fluid control. MiSA enables multiplexed applications like sequencing and droplet generation with high flexibility and reliability.

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Area of Science:

  • Life Sciences
  • Bioengineering
  • Microfluidics

Background:

  • Multiplexed fluid control is crucial but challenging in life sciences and bioengineering.
  • Existing systems can be costly and lack flexibility for diverse applications.

Purpose of the Study:

  • To present microfluidic sequence automation (MiSA), an open-source system for flexible, multiplexed fluid control.
  • To demonstrate MiSA's capability for stable flow, pulsed flow, and various microfluidic applications.

Main Methods:

  • MiSA integrates an Arduino Micro, ten solenoid valves, a pressure regulator, and a flow sensor for pressure-based feedback control.
  • A potentiometer is used to tune pressure control for stable microflow, especially at low flow rates.
  • The system was validated using hybridization-based in situ sequencing and extended for droplet generation, fiber spinning, and atomization.

Main Results:

  • MiSA provides 10-plex capability for constant flow and pulsed flow with millisecond precision.
  • The system demonstrated stable microflow control under low flow resistance.
  • Successful application in in situ sequencing, droplet generation, fiber spinning, and protein solution atomization.

Conclusions:

  • MiSA offers a cost-effective, reliable, and flexible solution for multiplexed microfluidic control.
  • The open-source nature of MiSA facilitates rapid exploration of microfluidic applications.
  • This system empowers researchers with advanced fluidic capabilities for diverse scientific endeavors.