Challenges in defining an efficient cleansing protocol against mono- and multi-species biofilms on a cobalt-chromium alloy surface
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Published on: December 9, 2022
Priscilla Neves Raile1, Patricia Almeida Curylofo1, Viviane de Cássia Oliveira1,2
1Department of Dental Materials and Prosthodontics, Dental School of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.
Background:
Effective cleaning protocols are crucial for controlling biofilm formation on oral prostheses and preserving the oral health of patients relying on removable partial dentures (RPDs).
Objectives:
The present study aimed to investigate the antibiofilm efficacy of 4 cleansing protocols on a cobalt-chromium (Co-Cr) alloy surface, which is commonly used as the base-metal framework material in dental prosthodontics.
Material And Methods:
Cobalt-chromium specimens were contaminated with isolated strains of Candida albicans, Candida glabrata, Staphylococcus aureus, and Streptococcus mutans to form monospecies biofilms. For a multi-species biofilm, all strains were grown simultaneously on the surfaces of the specimens. After biofilm maturation, the specimens were immersed in different solutions: Ricinus communis 2%; R. communis 10%; Cepacol™; NitrAdine™; and distilled water (control). After applying the hygiene protocols, the viability of the microorganisms and the amount of residual biofilm were assessed..
Results:
Immersion in R. communis-based solutions did not significantly alter the viability of the microorganisms. Cepacol reduced the viability of C. albicans, C. glabrata and S. aureus in the mono-species biofilms, as well as C. glabrata in the multi-species biofilm. NitrAdine demonstrated effectiveness in reducing the viability of C. glabrata and S. mutans in both the monoand multi-species biofilms. However, its efficacy against S. aureus was only observed in the mono-species pattern. NitrAdine also reduced the area covered by the living biofilm.
Conclusions:
The studied cleansing protocols exhibited reduced antimicrobial efficacy on the multispecies biofilm as compared to the mono-species model. NitrAdine showed potential as a complementary agent for controlling biofilm formation on removable partial dentures.
