Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Activation of Integrins01:15

Activation of Integrins

3.6K
Integrins bind ligands and transmit information from outside the cell to inside or vice-versa through an "outside-in signaling" or "inside-out signaling."
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding...
3.6K
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

2.8K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
2.8K
Integrins01:10

Integrins

4.1K
Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
4.1K
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

2.7K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
2.7K
Gastrulation01:56

Gastrulation

58.5K
Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...
58.5K
Anchoring Junctions01:03

Anchoring Junctions

3.9K
Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
3.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sex differences in the regulation and function of cellular immunity in Drosophila.

PLoS genetics·2026
Same author

A TRPV4-dependent calcium signaling axis regulates lamellipodial actin architecture to promote cell migration.

Current biology : CB·2026
Same author

Automated detection of spontaneous calcium signaling events in prohemocytes of the <i>Drosophila melanogaster</i> lymph gland.

microPublication biology·2025
Same author

Defining the role of integrins in melanoblast migration <i>in vivo</i>.

Molecular biology of the cell·2025
Same author

Talin autoinhibition is required for normal hemostasis.

Platelets·2025
Same author

Consolidation of cell-ECM adhesion through direct talin-mediated actin linkage is essential for mouse embryonic morphogenesis.

Communications biology·2025

Related Experiment Video

Updated: Sep 9, 2025

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes
09:14

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Published on: June 13, 2014

16.3K

Inside-out integrin activation is essential for early mammalian development.

Bhavya Venkatesh1, Angelica Phelan2, Guy Tanentzapf1

  • 1Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver V6T 1Z3, Canada.

Molecular Biology of the Cell
|September 3, 2025
PubMed
Summary

Precise regulation of integrin activity via talin is crucial for early mammalian development. Disrupting this inside-out activation leads to developmental defects and embryonic lethality, highlighting its role in cell survival and proliferation.

More Related Videos

Double Whole Mount in situ Hybridization of Early Chick Embryos
15:42

Double Whole Mount in situ Hybridization of Early Chick Embryos

Published on: October 27, 2008

12.5K
Dissection and Explant Culture of Murine Allantois for the In Vitro Analysis of Allantoic Attachment
09:30

Dissection and Explant Culture of Murine Allantois for the In Vitro Analysis of Allantoic Attachment

Published on: January 13, 2018

7.9K

Related Experiment Videos

Last Updated: Sep 9, 2025

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes
09:14

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Published on: June 13, 2014

16.3K
Double Whole Mount in situ Hybridization of Early Chick Embryos
15:42

Double Whole Mount in situ Hybridization of Early Chick Embryos

Published on: October 27, 2008

12.5K
Dissection and Explant Culture of Murine Allantois for the In Vitro Analysis of Allantoic Attachment
09:30

Dissection and Explant Culture of Murine Allantois for the In Vitro Analysis of Allantoic Attachment

Published on: January 13, 2018

7.9K

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Biochemistry

Background:

  • Cell-extracellular matrix (ECM) attachment is vital for morphogenesis.
  • Integrins mediate cell-ECM adhesion, and their activity requires precise regulation during development.
  • Inside-out activation, regulated by talin, is a key mechanism for integrin control.

Purpose of the Study:

  • To investigate the essential role of integrin activation modulation in early mammalian development.
  • To elucidate the function of talin-mediated inside-out integrin activation in embryonic development.

Main Methods:

  • Utilized mutations in the cytoplasmic protein talin to disrupt integrin activation.
  • Analyzed developmental phenotypes of mutant embryos and embryonic stem cells.
  • Assessed cell-ECM attachment strength, focal adhesion dynamics, cell survival, and proliferation.

Main Results:

  • Integrin activation mutants exhibit developmental delay, abnormal growth, and embryonic lethality by E8.5-9.5.
  • Disruption of integrin regulation did not affect embryonic patterning or ECM distribution.
  • Mutant cells showed reduced cell-ECM attachment strength with mild defects in focal adhesion.
  • Increased cell death and reduced cell proliferation were observed in activation mutants at E7.5.

Conclusions:

  • Inside-out integrin activation, mediated by talin, strengthens cell-ECM attachment in early mouse development.
  • This process is essential for embryonic cell survival and proliferation.
  • Precise regulation of integrin activity is critical for successful mammalian development.