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Updated: Sep 9, 2025

Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells
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PRAME Expression in Recurrent/Persistent Melanocytic Nevi.

Alison H Kucharik1, Helana Ghali2, Doniya Milani1,3

  • 1Department of Dermatology and Cutaneous Surgery, University of South Florida, Tampa, FL.

The American Journal of Dermatopathology
|September 3, 2025
PubMed
Summary
This summary is machine-generated.

PRAME immunohistochemistry is useful for diagnosing recurrent melanocytic nevi. This stain helps differentiate benign nevi from melanoma, improving diagnostic confidence and potentially reducing unnecessary procedures.

Keywords:
PRAMErecurrent nevus

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Area of Science:

  • Dermatopathology
  • Immunohistochemistry
  • Oncology

Background:

  • Recurrent melanocytic nevi can present with atypical features, complicating diagnosis, especially without prior biopsy information.
  • The PReferentially expressed Antigen in MElanoma (PRAME) stain is used for melanoma diagnosis, but its role in recurrent nevi is unclear.

Purpose of the Study:

  • To evaluate the utility of PRAME immunohistochemistry in distinguishing benign recurrent melanocytic nevi from melanoma.
  • To assess PRAME expression consistency between original and recurrent nevi.

Main Methods:

  • Analyzed PRAME expression in paired original and recurrent melanocytic nevi from 22 patients.
  • Evaluated PRAME immunohistochemistry using staining intensity, percentage of positive melanocytes, and a composite score.

Main Results:

  • PRAME expression patterns were consistent between original and recurrent nevi.
  • All analyzed cases, both original and recurrent nevi, showed negative composite scores for PRAME staining.

Conclusions:

  • PRAME immunohistochemistry demonstrates utility in differentiating benign recurrent nevi from melanoma.
  • Consistent negative PRAME composite scores in recurrent nevi enhance diagnostic confidence and may help avoid unnecessary procedures.