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Updated: Sep 9, 2025

Novel Production Protocol for Small-scale Manufacture of Probiotic Fermented Foods
Published on: September 10, 2016
Endogenous biogeneration of prebiotic functional ingredients in dairy products using levansucrases
Wong Min Muriel1, Salwa Karboune1
1McGill University, Department of Food Science and Agricultural Chemistry, 21111 Lakeshore, Ste-Anne-de-Bellevue, Quebec H9X 3V9, Canada.
Abstract:
This study focuses on the application of levansucrases (LS, EC 2.4.1.10), from Gluconobacter oxydans (LS1), Vibrio natriegens (LS2), Novosphingobium aromaticivorans (LS3), and Paraburkholderia graminis (LS4), in dairy products. The effects of pH and temperature on lactose transfructosylation were first evaluated. Overall, transfructosylation was predominant over hydrolysis for G. oxydans LS1, V. natriegens LS2, and P. graminis LS4. V. natriegens LS2 showed the highest potential, with high lactosucrose production even at the pH of milk and at a low temperature. A diversified range of fructooligosaccharides (FOS) was detected with N. aromaticivorans LS3 and P. graminis LS4. Then, the selected LSs were applied to sweetened milk and chocolate milk. No significant changes were observed in the reaction selectivity, sucrose conversion, lactosucrose and FOS production when the chocolate milk formulations were enriched with additional cocoa powder. Finally, levan from G. oxydans LS1 proved to be a potential stabilizer of great interest in chocolate milk production.
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