Related Experiment Video
Updated: Jun 17, 2026

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
Published on: April 17, 2017
Comparative profiling of splenic phospholipid and sphingolipid during acute infection
Tao Liu1, Hong Jia2, Hui Dong3
1College of Animal Science and Technology, Xinyang Agriculture and Forestry University, Xinyang, 464000, China.
Abstract:
Lipid profile of spleen and bursa of Fabricius (BF) during acute infection remains unknown. Acute infection models of porcine reproductive and respiratory syndrome virus (PRRSV), porcine epidemic diarrhea virus (PEDV) and Eimeria tenella (ET) were developed, and spleen samples with African swine fever virus (ASFV) or not were collected. Spleen and BF were examined and characteristic microscopic lesions were observed. Samples were analyzed using a semi-quantitative, untargeted method based on liquid chromatography-electrospray ionization tandem mass spectrometry (LC-MS-MS). After statistical analysis, potential biomarkers for PRRSV [16:0 20:5 phosphatidylcholine (16:0 20:5 PC), 18:1 lysophosphatidylcholine (18:1 LPC)], for PEDV [16:0 20:4 phosphatidylinositol (16:0 20:4 PI), 18:0 18:2 phosphatidylserine (18:0 18:2 PS), 16:0 lysophosphatidylserine (16:0 LysoPS), 18:0 LysoPS, d18:1 16:0 sphingomyelin (d18:1 16:0 SM)], for ASFV [16:0 18:0 PC, 16:0 20:4 PC, 18:0 20:4 PC, O-16:0 20:4 PC, O-18:1 20:4 PC, 16:0 18:2 phosphatidylethanolamine (16:0 18:2 PE), 16:0 18:1 PE, 18:0 20:4 PE, 16:0 18:2 PI, 18:0 18:1 PS], for ET with spleen [16:0 20:3 PC, 18:0 18:2 PC, 18:0 18:1 PI, 18:0 20:4 PI, 18:0 22:6 PI, d18:1 16:0 SM, d18:1 22:1 SM], for ET with BF [16:0 18:2 PI, 16:0 18:1 PI, 18:0 18:2 PI, 18:0 20:4 PI, 16:0 18:2 PS, 16:0 18:1 PS, 18:0 18:2 PS, 18:0 20:4 PS, 18:0 22:6 PS, 18:0 LysoPS, 20:4 arachidonic acid (20:4 AA), 22:6 docosahexaenoic acid (22:6 DHA)] were analyzed. Overall, lipid profiling of spleen and BF was demonstrated, contributing to understand the lipid biology of lymphoid organs and intervene acute infection.
More Related Videos
08:38Using a Bacterial Pathogen to Probe for Cellular and Organismic-level Host Responses
Published on: February 22, 2019
11:59Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids
Published on: May 15, 2019
Related Concept Videos
Acute Pharyngitis
Acute pharyngitis is the inflammation of the back of the throat (pharynx), commonly resulting in a sore throat. It is a frequently encountered condition that prompts individuals to seek medical advice.
Classification
Acute pharyngitis can be categorized based on its underlying cause:
Acute Inflammation I: Inflammatory Response
Acute Inflammation II: Cellular Phase
Acute Inflammation III: Local and Systemic Effects
Encephalitis ll: Pathophysiology
Acute Pancreatitis II: Pathophysiology