Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

527
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
527
Electrophoresis: Overview01:20

Electrophoresis: Overview

2.2K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
2.2K
Effects of EDTA on End-Point Detection Methods01:18

Effects of EDTA on End-Point Detection Methods

344
Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
344
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

375
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
375

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparing capillary coatings for protein separation by capillary electrophoresis.

Analytica chimica acta·2026
Same author

Determination of the concentration and size-distribution of residual capsular poly(N-acetyl neuraminic acid) from outer membrane vesicle vaccines by micellar electrokinetic capillary chromatography.

Analytica chimica acta·2026
Same author

Critical parameters for achieving high efficiency and reproducible double-chained cationic surfactant coatings for protein separation by capillary electrophoresis.

Journal of chromatography. A·2026
Same author

Protein Amyloid and Synthetic Polymer Self-Assemblies: Similar Challenges for Their Preparation Associated with Shared Properties.

Biomacromolecules·2026
Same author

Evaluation of zwitterionic silica monolayer as new coating for the separation of intact proteins by capillary electrophoresis.

Journal of chromatography. A·2025
Same author

Clinical Pharmacokinetic Assessment of Lazertinib in Healthy Adult Participants: Effects of GSTM1 Genotype.

Clinical drug investigation·2025

Related Experiment Video

Updated: Sep 9, 2025

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
07:46

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Published on: October 1, 2016

11.8K

Interaction study between gadolinium-based contrast agents and lysozyme using frontal analysis continuous capillary

Chutintorn Somnin1, Joseph Chamieh1, Laurent Leclercq1

  • 1IBMM, University of Montpellier, CNRS, ENSCM, 34095, Montpellier, France.

Analytica Chimica Acta
|September 3, 2025
PubMed
Summary

Gadolinium-based contrast agents (GBCAs) show varying cooperative binding to lysozyme protein. Frontal Analysis Continuous Capillary Electrophoresis (FACCE) effectively analyzes these interactions, revealing binding strengths and stoichiometry for improved MRI contrast agent safety.

Keywords:
Frontal analysis continuous capillary electrophoresisGadolinium-based contrast agentsInteractionlysozyme

More Related Videos

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
10:05

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

9.6K
Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time
11:05

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time

Published on: October 26, 2016

9.2K

Related Experiment Videos

Last Updated: Sep 9, 2025

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
07:46

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Published on: October 1, 2016

11.8K
Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
10:05

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

9.6K
Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time
11:05

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time

Published on: October 26, 2016

9.2K

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Pharmacology

Background:

  • Gadolinium-based contrast agents (GBCAs) are crucial for magnetic resonance imaging (MRI).
  • Understanding GBCA interactions with biological macromolecules is vital for safety and next-generation agent design.
  • Robust analytical methods are needed to study these interactions.

Purpose of the Study:

  • To investigate the binding interactions between lysozyme and various GBCAs using Frontal Analysis Continuous Capillary Electrophoresis (FACCE).
  • To determine the stoichiometry, binding strength, and cooperativity of these interactions.
  • To assess the suitability of FACCE for analyzing GBCA-protein interactions.

Main Methods:

  • Frontal Analysis Continuous Capillary Electrophoresis (FACCE) was employed.
  • Lysozyme was used as a model protein.
  • Several GBCAs were tested: Gd-DTPA, Gd-BOPTA, Gd-DOTA, Gd-PCTA D2, and Gd-HP-DO3A.

Main Results:

  • GBCAs exhibited varying degrees of cooperative binding to lysozyme.
  • Stoichiometry (n=4) was observed for Gd-DTPA, Gd-BOPTA, and Gd-DOTA.
  • Binding strength followed the order: Gd-BOPTA > Gd-DTPA > Gd-DOTA. Gd-PCTA D2 showed no significant binding.
  • FACCE could not resolve interactions for Gd-HP-DO3A due to interference.

Conclusions:

  • FACCE is a suitable method for studying GBCA-protein interactions, requiring minimal sample volume.
  • The method allows determination of stoichiometry, binding constants, and cooperativity.
  • GBCA-lysozyme interactions are cooperative, and the Hill model effectively analyzes them within the tested concentration range.