Cytophotometric determination of basic proteins with heparin-alcian blue method

For the quantitative cytochemical determination of basic proteins, Heparin-Alcian Blue (HAB-was found to be a more suitable staining procedure than the generally used alcalic Fast green reaction. HAB is able to stain all the histone fractions, but there is no staining following deamination (gelelectrophoretic examinations) and no aspecific staining (Millipore filter model). In EHRLICH ascites tumour cells, changes in the FEULGEN-DNA content is followed by the proportional changes of HAB-basic protein content. After HAB reaction, the FEULGEN reaction can be performed on the same slide.