Organoid-Based Skin and Lung Biofilm Models, a Cutting-Edge Approach for Anti-Biofilm Research: A Mini Review

Bacterial biofilms contribute to 60%-80% of human infections, exhibiting resistance to traditional antibiotic treatment and contributing to chronic, relapsing diseases, particularly in healthcare settings. Traditional in-vitro and in-vivo models often fail to accurately replicate the human microenvironment. This mini review highlights the emerging use of organoid-based models that are three-dimensional, self-organizing structures derived from stem cells. These biomimetic systems closely resemble native organs, providing a physiologically appropriate platform for anti-biofilm efficacy assessment, especially skin and lung, offering a more accurate environment for assessing microbial colonization, persistence, and therapeutic response. This paper summarizes recent advances in the development of effective antimicrobial testing methods for biofilm organoid models, focusing on human-derived proteins and biopolymers. We have discussed how these organoid models, specifically skin and lung organoids, provide insights into host-pathogen dynamics and antimicrobial responses. By bridging the gap between the clinical phase and classical experimental modeling, the organoid model is a powerful tool for transforming and accelerating translational antimicrobial research.