Eco-Friendly Silver Nanoparticles Synthesis Method Using Medicinal Plant Fungal Endophytes-Biological Activities and...

Area of Science:

• Biomedical Nanotechnology
• Green Synthesis
• Myco-fabrication

Background:

• Nanotechnology and green synthesis offer innovative biomedical solutions.
• Endophytic fungi present a sustainable source for nanoparticle biofabrication.
• Bergenia ciliata harbors endophytic fungi with potential bioactivity.

Purpose of the Study:

• To biofabricate silver nanoparticles (AgNPs) using the endophytic fungus Corynespora smithii.
• To evaluate the biological activities of the synthesized AgNPs.
• To assess the therapeutic potential of a key bioactive compound from C. smithii.

Main Methods:

• Green synthesis of AgNPs using Corynespora smithii fungal extracts.
• Characterization of AgNPs and assessment of antioxidant, antibacterial, and anticancer properties.
• Molecular docking and ADME analysis of the bioactive compound dimethylsulfoxonium formylmethylide.

Main Results:

• Eco-friendly synthesis of AgNPs with significant antioxidant, antibacterial, and anticancer activities.
• Demonstrated efficacy against Gram-positive and Gram-negative bacteria.
• Cytotoxicity against A549 lung cancer cells (IC50 = 10.46 µg/mL).
• Favorable pharmacokinetic profile for dimethylsulfoxonium formylmethylide, suggesting oral bioavailability.

Conclusions:

• Corynespora smithii is a viable source for sustainable AgNP synthesis with therapeutic potential.
• Bioactive compounds from endophytic fungi can be developed into novel therapeutic agents.
• This study supports the advancement of nanotechnology in biomedicine through endophytic fungi.