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Updated: Sep 9, 2025
Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
Published on: October 4, 2019
Circulating MicroRNA Expression in Glioblastoma: A Promising Diagnostic Panel
Vincenzo Macaione1, Francesca Graziano2, Francesca Polito3
1Department of Clinical and Experimental Medicine, University of Messina, Messina, Italy, vmacaione@unime.it.
Introduction:
Among malignant brain tumors, glioblastoma (GB) is one of the most common. An early diagnosis would benefit the prognosis: in this regard, the search for sensitive and specific biomarkers is particularly active. In fact, liquid biopsy has been proposed by many researchers as a valid and repeatable method of studying GB. Among the biomarkers researched in blood patients, microRNAs (miRNAs) are among the most encouraging. The aim of our research was to select blood miRNAs overexpressed in GB patients to establish a reliable biomarker panel for pathology.
Methods:
We studied blood expression of 14 miRNAs in 30 subjects with GB, before and after surgery, and 30 healthy controls.
Results:
Statistical analysis allowed us to select a panel of 3 miRNAs (miR-340-5p, miR-369-3p, miR-486-5p) that are overexpressed in GB patients.
Conclusion:
Our results showed that simultaneous measurement of circulating miR-340-5p, miR-369-3p, and miR-486-5p represents a promising biomarker for the diagnosis and follow-up of GB patients.
