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Related Experiment Video

Updated: Apr 28, 2026

Fluorescence detection methods for microfluidic droplet platforms
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Rapid and practical microbial detection method for brewery hygiene management using the EZ-Fluo system.

Maika Kitazawa1, Nobuchika Takesue2, Kazumaru Iijima2

  • 1R&D Institute for Product Development, Asahi Breweries, Ltd., 1-21 Midori 1-Chome, Moriya, Ibaraki 302-0106, Japan.

Journal of Bioscience and Bioengineering
|September 4, 2025
PubMed
Summary

Rapidly detect brewery microorganisms with the EZ-Fluo Rapid Detection System. This optimized method provides results in 30 hours, significantly improving hygiene management and reducing contamination risks compared to traditional culture methods.

Keywords:
BreweryCarboxyfluorescein diacetateEZ-Fluo Rapid Detection SystemHygiene testMicrobial testMicrocolony methodSwab testing

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Area of Science:

  • Food Microbiology
  • Industrial Biotechnology
  • Analytical Chemistry

Background:

  • Microbial contamination poses significant risks to brewery operations, impacting product quality and safety.
  • Traditional culture-based hygiene tests are time-consuming, delaying corrective actions.
  • Rapid and reliable detection methods are essential for effective brewery hygiene management.

Purpose of the Study:

  • To optimize test conditions for the EZ-Fluo Rapid Detection System for brewery hygiene.
  • To evaluate the performance of the optimized EZ-Fluo system against traditional methods.
  • To establish a cost-effective and efficient method for early microorganism detection in breweries.

Main Methods:

  • Optimization of incubation temperature, fluorescent reagent, and reaction temperature for the EZ-Fluo system.
  • Testing with environmental indicator species Acetobacter pasteurianus and Pseudomonas fluorescens.
  • Validation using brewery hygiene samples and comparison with traditional culture methods.

Main Results:

  • The optimized EZ-Fluo system (EZF-ODC method) detected nine common brewery indicator species with 54.0-117.2% recovery after 30 hours.
  • The EZF-ODC method showed comparable results to traditional 72-hour culture methods, with 94.6% agreement in 222 samples.
  • No significant variation in colony-forming units was observed among analysts, indicating method reliability.

Conclusions:

  • The EZF-ODC method offers a significant improvement over traditional methods, reducing detection time from 72 hours to 30 hours.
  • This rapid detection enables timely implementation of hygiene control measures, ensuring a cleaner brewery environment.
  • The EZ-Fluo system provides a cost-effective solution for enhanced brewery hygiene management and potentially expedited production planning.