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Updated: May 3, 2026

DNA Methylation: Bisulphite Modification and Analysis
Published on: October 21, 2011
RoAM: computational reconstruction of ancient methylomes and identification of differentially methylated regions
Yoav Mathov1,2, Naomi Rosen1, Chen Leibson1
1Department of Genetics, The Alexander Silberman Institute of Life Sciences, Faculty of Science, the Hebrew University of Jerusalem, Jerusalem, 9190401, Israel.
Abstract:
We present a new and considerably improved version of RoAM (Reconstruction of Ancient Methylation), a flexible tool for reconstructing ancient methylomes and identifying differentially methylated regions (DMRs) between populations. Through a series of filtering and quality control steps, RoAM produces highly reliable DNA methylation maps, making it a valuable tool for paleoepigenomics studies. We apply RoAM to pre-and post-Neolithic transition Balkan samples, and uncover DMRs in genes related to sugar metabolism. Notably, we observe post-Neolithic hypermethylation of PTPRN2, a regulator of insulin secretion. These results are compatible with hypoinsulinism in pre-Neolithic hunter-gatherers.
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