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DNA Dynamics in Dual Nanopore Tug-of-War.

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  • 1Department of Physics, McGill University, Montréal, Québec.

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This study explores DNA translocation through dual nanopores using a tug-of-war method. Longer DNA molecules can be trapped, and their escape dynamics reveal key physics for controlled single-molecule sensing.

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Area of Science:

  • Nanotechnology
  • Biophysics
  • Molecular Biology

Background:

  • Solid-state nanopores are vital for single-molecule sensing.
  • Uncontrolled, rapid molecule translocation through nanopores is a major challenge.
  • Previous work showed dual-nanopore systems offer controlled translocation via feedback biasing.

Purpose of the Study:

  • To investigate the physics of DNA translocation in a dual-nanopore tug-of-war configuration.
  • To analyze the behavior of longer, genomically relevant DNA molecules (T4-DNA, 166 kbp).
  • To understand the dynamics of DNA disengagement from the tug-of-war state.

Main Methods:

  • Utilizing a dual-nanopore system with feedback-controlled biasing for DNA capture.
  • Applying opposing electrophoretic forces to DNA molecules co-captured in a tug-of-war.
  • Systematically varying pore voltage, DNA size, and translocation configurations.

Main Results:

  • Longer DNA molecules can be trapped in asymmetric tug-of-war states.
  • The velocity of DNA free-end escape depends on pore voltage and DNA size.
  • Observed dynamics validate theoretical predictions from a first passage model.

Conclusions:

  • Dual-nanopore tug-of-war enables controlled translocation of longer DNA molecules.
  • Understanding DNA disengagement physics is crucial for advanced nanopore sensing.
  • This research provides insights into molecular dynamics at the nanoscale.