Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Necrosis01:16

Necrosis

4.8K
Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”.
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...
4.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cell size modulates ferroptosis susceptibility.

eLife·2026
Same author

Discovery of a paralog-selective p300 protein degrader with potent anti-cancer activity in hematological malignancies.

Nature communications·2026
Same author

Dissecting Complex Interactions Between Ferroptosis and the Proteasome.

bioRxiv : the preprint server for biology·2026
Same author

Recurrent Genomic Alterations in BCRi-Experienced CLL Patients Treated With Venetoclax: Extended Phase 2 Follow-Up.

American journal of hematology·2026
Same author

Cell size modulates ferroptosis susceptibility.

bioRxiv : the preprint server for biology·2025
Same author

A guide to using small-molecule ferroptosis inhibitors.

Nature structural & molecular biology·2025
Same journal

CDK2 Inhibition Exerts RB-Independent Antitumor Activity in CDK4/6 Inhibitor-Resistant HR+/HER2- Breast Cancer.

Cancer research·2026
Same journal

A Clinically Integrated Pediatric Patient-Derived Xenograft Program Enables Evaluation of Cohort and Patient-Specific Biology and Therapeutic Strategies.

Cancer research·2026
Same journal

Editor's Note: Heterodimerization of Insulin-like Growth Factor Receptor/Epidermal Growth Factor Receptor and Induction of Survivin Expression Counteract the Antitumor Action of Erlotinib.

Cancer research·2026
Same journal

Editor's Note: Deguelin Analogue SH-1242 Inhibits Hsp90 Activity and Exerts Potent Anticancer Efficacy with Limited Neurotoxicity.

Cancer research·2026
Same journal

Retraction: Two Functional Epitopes of Pigment Epithelial-Derived Factor Block Angiogenesis and Induce Differentiation in Prostate Cancer.

Cancer research·2026
Same journal

Editor's Note: Chronic Stress Facilitates Lung Tumorigenesis by Promoting Exocytosis of IGF2 in Lung Epithelial Cells.

Cancer research·2026
See all related articles

Related Experiment Video

Updated: Sep 8, 2025

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics
04:01

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics

Published on: March 15, 2024

1.2K

Lipid Composition Alters Ferroptosis Sensitivity.

Vivian S Park1, Lauren E Pope2, Justin P Ingram1

  • 1AbbVie Inc., North Chicago, Illinois.

Cancer Research
|September 5, 2025
PubMed
Summary
This summary is machine-generated.

Ferroptosis, a cell death process, is resisted by cancer cells in 3D cultures and tumors. Lipid changes in these conditions reduce sensitivity to GPX4 inhibitors, impacting drug development.

More Related Videos

Ferritinophagy: Assessing the Selective Degradation of Iron by Autophagy in Human Fibroblasts
09:21

Ferritinophagy: Assessing the Selective Degradation of Iron by Autophagy in Human Fibroblasts

Published on: February 23, 2024

1.0K
Assessing Iron Deposition in the Brains of 5xFAD Mice by Perls'/DAB Staining
07:32

Assessing Iron Deposition in the Brains of 5xFAD Mice by Perls'/DAB Staining

Published on: May 23, 2025

458

Related Experiment Videos

Last Updated: Sep 8, 2025

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics
04:01

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics

Published on: March 15, 2024

1.2K
Ferritinophagy: Assessing the Selective Degradation of Iron by Autophagy in Human Fibroblasts
09:21

Ferritinophagy: Assessing the Selective Degradation of Iron by Autophagy in Human Fibroblasts

Published on: February 23, 2024

1.0K
Assessing Iron Deposition in the Brains of 5xFAD Mice by Perls'/DAB Staining
07:32

Assessing Iron Deposition in the Brains of 5xFAD Mice by Perls'/DAB Staining

Published on: May 23, 2025

458

Area of Science:

  • Cellular Biology
  • Biochemistry
  • Oncology

Background:

  • Ferroptosis is an iron-dependent cell death marked by lipid peroxidation.
  • Glutathione peroxidase 4 (GPX4) inhibits ferroptosis by reducing lipid hydroperoxides.
  • GPX4 is a potential drug target, necessitating understanding of inhibitor sensitivity.

Purpose of the Study:

  • Investigate factors influencing GPX4 inhibitor sensitivity in cancer.
  • Determine the impact of 3D culture and in vivo conditions on ferroptosis resistance.
  • Identify mechanisms of altered ferroptosis sensitivity.

Main Methods:

  • Compared ferroptosis induction by GPX4 reduction in 2D vs. 3D cancer cell cultures.
  • Assessed GPX4 inhibitor efficacy in 2D cultures, 3D spheroids, and mouse xenografts.
  • Analyzed lipidomic changes, specifically monounsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs), using gene expression of stearoyl-CoA desaturase (SCD).

Main Results:

  • Reduced GPX4 expression induced ferroptosis in 2D cultures but not consistently in mouse xenografts.
  • 3D spheroid culture conferred resistance to GPX4 inhibition compared to 2D.
  • 3D growth and in vivo tumors showed increased MUFA-PLs and decreased PUFA-PLs, linked to SCD upregulation, promoting ferroptosis resistance.

Conclusions:

  • Cancer cell adaptation to 3D and in vivo environments, involving lipidome remodeling, confers resistance to GPX4 inhibitors.
  • Altered MUFA/PUFA ratios are a key mechanism limiting GPX4 inhibitor efficacy.
  • Findings suggest strategies to overcome resistance to ferroptosis-inducing drugs.