Related Experiment Video
Updated: Sep 8, 2025

Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells
Published on: April 4, 2016
Wadjet-Keeping a watchful eye on circular DNA
1Department of Cellular & Molecular Medicine, University of California, San Diego, La Jolla, CA, USA; Department of Molecular Biology, University of California, San Diego, La Jolla, CA, USA.
Abstract:
The structural maintenance of chromosomes (SMC)-family Wadjet complex restricts plasmid transformation in bacteria through a distinctive mechanism coupling DNA loop extrusion and cleavage. In this issue of Structure, Roisné-Hamelin et al.1 report the biochemical reconstitution and structure of a type II Wadjet complex, revealing a shared overall mechanism and notable architectural differences compared to related type I complexes.

