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Updated: Jun 26, 2026

Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors
Published on: April 13, 2019
Noninvasive multiclass milk contaminant detection using hyperspectral imaging and hybrid ensemble learning
Muhammad Iqbal1, Muhammad Aqeel2, Ahmed Sohaib2
1School of Interdisciplinary Engineering and Sciences (SINES), National University of Sciences and Technology (NUST), Islamabad 44000, Pakistan.
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Food contamination remains a serious global concern due to its health risks, with milk being one of the most commonly adulterated foods in developing countries such as Pakistan, India, and Bangladesh. Accurate detection of milk contamination is essential for ensuring consumer safety and maintaining food industry standards. This study explores both invasive and noninvasive approaches for contamination analysis. The invasive method uses the Lactoscan system to assess parameters such as fat, conductivity, protein, density, solids, lactose, temperature, pH, and SNF across varying contamination levels. The noninvasive method employs hyperspectral imaging using the Specim FX-10 system (400-1,000 nm) to detect contamination through spectral and spatial analysis. Preprocessing involved image resizing and region of interest selection for feature extraction, as well as radiometric correction using the empirical line method. Postprocessing included noise reduction and spectral smoothing using the Savitzky-Golay filter. The resulting clean spectral data were classified using a hybrid ensemble learning (HEL) framework, which combines voting and stacking ensembles of gradient boosting, XGBoost, LightGBM, and multilayer perceptron models. Comparative results show the HEL approach significantly outperforms existing methods, achieving 100% training and 96% validation accuracy-demonstrating its potential for real-time, noninvasive milk quality assurance.

