Resolving leukemic stem cell heterogeneity and plasticity with single-cell multiomics

Area of Science:

• Hematology
• Cancer Biology
• Genomics

Background:

• Acute myeloid leukemia (AML) is driven by diverse leukemic stem cells (LSCs).
• LSC heterogeneity impacts therapy response and disease relapse.
• Understanding LSC diversity is crucial for effective AML treatment.

Purpose of the Study:

• To review the causes and consequences of LSC heterogeneity in AML.
• To highlight advances in single-cell multiomics for dissecting LSC diversity.
• To explore how these technologies can improve AML diagnostics and monitoring.

Main Methods:

• Review of current scientific literature.
• Focus on single-cell multiomics technologies (genomics, transcriptomics, epigenomics, proteomics).
• Analysis of LSC heterogeneity and plasticity in AML.

Main Results:

• LSC heterogeneity arises from genetic and non-genetic factors.
• Single-cell multiomics provides high-resolution molecular snapshots of individual LSCs.
• This technology enables a deeper understanding of LSC states and vulnerabilities.

Conclusions:

• LSC heterogeneity presents challenges but also therapeutic opportunities in AML.
• Single-cell multiomics is transforming the study of AML LSC diversity.
• Advances in these technologies promise to revolutionize AML diagnostics and patient monitoring.