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Updated: Jun 4, 2026

Surgical Retrieval, Isolation and In vitro Expansion of Human Anterior Cruciate Ligament-derived Cells for Tissue Engineering Applications
Published on: April 30, 2014
[Augmented reconstruction of the posterior cruciate ligament for acute and chronic posterior instability]
Svenja Höger1, Sebastian Siebenlist2, Julian Mehl1
1Sektion Sportorthopädie, TUM Universitätsklinikum , Ismaninger Str. 22, 81675, München, Deutschland.
Objective:
Anatomical reconstruction of the posterior cruciate ligament (PCL) with suture tape augmentation to enhance primary stability.
Indications:
Acute or chronic PCL ruptures, either isolated or as part of multiligamentous injuries, in cases of symptomatic instability or failure of conservative treatment.
Contraindications:
Fixed posterior drawer, active infection, bony avulsion.
Surgical Technique:
Supine positioning, use of standard portals for arthroscopic PCL reconstruction. Femoral and tibial tunnels are created using a targeting device following preparation of the insertion sites. The hamstring graft is augmented with nonresorbable suture tape. The augmentation suture is fixed separately using a suture anchor under continuous anterior drawer stress. Graft fixation is performed via extracortical device at the femur and with an interference screw at the tibia.
Postoperative Management:
Six weeks of extension bracing with posterior tibial support, worn 24 h/day, and 20 kg partial weight-bearing. This is followed by 6 weeks of continued mobile bracing with posterior tibial support and progressive mobilization and load increase, accompanied by physiotherapy.
Results:
Biomechanical and early clinical studies suggest promising benefits of augmentation for stability and function, though robust long-term data are still lacking.
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