Development of a Sensitive Fluorimetric Method for Atomoxetine Determination in Tablets and Plasma Using a Dansyl...

Area of Science:

• Analytical Chemistry
• Green Chemistry
• Pharmaceutical Analysis

Background:

• Atomoxetine (AXT) analysis is crucial for therapeutic drug monitoring and quality control.
• Existing analytical methods may lack environmental sustainability or sensitivity.
• Development of eco-friendly and robust analytical techniques is an ongoing need.

Purpose of the Study:

• To develop and validate a novel, environmentally friendly method for atomoxetine (AXT) quantification.
• To utilize dansyl chloride as a fluorescent derivatizing agent for enhanced detection.
• To assess the method's performance and sustainability profile according to international guidelines.

Main Methods:

• Fluorescence derivatization of atomoxetine (AXT) using dansyl chloride.
• Quantitative analysis using validated analytical instrumentation.
• Method validation according to International Conference on Harmonization (ICH) guidelines.
• Sustainability assessment using multiple green chemistry metrics (RGB12, Green Certificate, AGREE, SPMS, BAGI).

Main Results:

• The method demonstrated excellent linearity (r² = 0.9995) over a wide range (50.0–900.0 ng/mL).
• High accuracy (99.56%–100.67%) and precision (98.87%–102.54%) were achieved.
• Successful application to pharmaceutical formulations and biological matrices (plasma).
• Comprehensive sustainability profiling indicated a favorable environmental impact.

Conclusions:

• The proposed dansyl chloride-based method provides a sensitive, accurate, and precise approach for atomoxetine (AXT) analysis.
• The method is validated and suitable for real-world applications in pharmaceutical and clinical settings.
• Its green chemistry profile makes it an attractive alternative to conventional analytical techniques.