Multi-frequency electromagnetic radiation promoted hepatic lipid metabolism via the adiponectin pathway in mice

Radiofrequency electromagnetic radiation (RF-EMR) is ubiquitous in daily life, and the complexity of the electromagnetic environment continues to increase. Nevertheless, research on the biological effects of compound radiofrequency radiation-particularly its influence on lipid metabolism under realistic exposure scenarios-remains limited, and the underlying mechanisms are not yet fully elucidated. To investigate the impact of compound radiofrequency radiation on lipid metabolism, multi-frequency radiation exposure model both normal and obese mice were established. Our findings indicated that prolonged exposure to composite three-frequency radiation led to a significant reduction in serum levels of TG and LDL, accompanied by an increase in HDL. Concurrently, we observed upregulation of UCP1 expression and enhanced lipid metabolic activity in adipose tissue. Furthermore, radiation exposure promoted the secretion of adiponectin and other adipokines from adipose tissue. These adipokines subsequently activated the adiponectin signaling pathway in the liver, stimulated fatty acid β-oxidation while suppressed lipogenesis. In the obesity model, long-term exposure to compound three-frequency radiation similarly enhanced lipid metabolism in adipose tissue and stimulated adiponectin secretion. This subsequently activated the adiponectin signaling pathway in the liver, led to improved hepatic lipid homeostasis and a reduction in the severity of both hepatic steatosis and fibrosis. Taken together, these findings demonstrated that compound radiofrequency radiation enhanced lipid metabolism in adipose tissue and liver through adiponectin-mediated mechanisms, thereby promoted systemic metabolic regulation.