Related Experiment Video

Updated: Jan 18, 2026

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer
09:40

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

3.2K

GJB2 promotes ovarian cancer progression and cisplatin resistance by upregulating TNC expression

Jiaxuan Zhao1, Yunfei Lu1, Kun Yang1

  • 1Department of Clinical Laboratory, North China University of Science and Technology Affiliated Tangshan Maternal and Child Health Care Hospital-Tangshan, China; Key Laboratory of Molecular Medicine for Abnormal Development and Related Diseases in Tangshan City-Tangshan, China.

Biochimica Et Biophysica Acta. Molecular Cell Research
|September 7, 2025
PubMed

Insights

Gap junction protein β-2 (GJB2) drives ovarian cancer progression and cisplatin resistance by increasing tenascin C (TNC) expression. Inhibiting GJB2 with epicatechin may overcome chemotherapy resistance.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Cisplatin resistance is a significant challenge in ovarian cancer (OC) treatment.
  • Gap junction protein β-2 (GJB2), linked to hereditary deafness, has an unexplored role in OC chemotherapy resistance.

Purpose of the Study:

  • To investigate the role of GJB2 in ovarian cancer progression and cisplatin resistance.
  • To explore the relationship between GJB2, tenascin C (TNC), and epicatechin in OC.

Main Methods:

  • Assessed GJB2 expression in OC patients and cell lines.
  • Performed GJB2 knockdown and overexpression experiments in OVCAR-3 cells.
  • Analyzed the correlation between GJB2 and TNC in clinical samples.
  • Investigated the effect of epicatechin on GJB2 expression and cisplatin sensitivity.

Main Results:

  • Increased GJB2 expression correlated with poor prognosis and cisplatin resistance in OC.
  • GJB2 knockdown enhanced cisplatin sensitivity and inhibited OC progression.
  • GJB2 overexpression promoted OC progression and resistance.
  • A positive correlation between GJB2 and TNC was observed.
  • TNC knockdown reversed GJB2-driven OC progression and resistance.
  • Epicatechin increased cisplatin sensitivity by inhibiting GJB2 expression.

Conclusions:

  • GJB2 is a potential therapeutic target for overcoming cisplatin resistance in ovarian cancer.
  • GJB2 may promote OC progression and resistance by upregulating TNC expression.
  • Epicatechin shows potential to enhance cisplatin efficacy by targeting GJB2.
Keywords:
Cisplatin resistanceEpicatechinGJB2Ovarian cancerTNC

More Related Videos

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer09:08

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer

Published on: January 12, 2020

7.2K
Author Spotlight: Exploring the Role of FAM83A in Cervical Cancer04:20

Author Spotlight: Exploring the Role of FAM83A in Cervical Cancer

Published on: February 9, 2024

1.4K

Related Concept Videos

Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
9.4K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.7K
Tumor Progression02:07

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
7.2K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.6K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
5.4K
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
5.9K