Related Experiment Video
Updated: Jan 18, 2026

Hypoxic Preconditioning of Marrow-derived Progenitor Cells As a Source for the Generation of Mature Schwann Cells
Published on: June 14, 2017
Senescence-regulating agents remodel mesenchymal stem cell-schwann cell circuitry for diabetic bone regeneration
An Lao1, Yiting Sun2, Weiqi Li3
1Department of Stomatology, Xin Hua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China; Department of Oral and Cranio-maxillofacial Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, 200011, China.
Abstract:
Bone healing requires Schwann cells (SCs) paracrine factors for mesenchymal stem cell function. Diabetes mellitus (DM) patients are susceptible to developing SCs dysfunction and impairing bone healing. Rare research considered reconstructing mesenchymal stem cell-schwann cell circuitry in diabetic bone regeneration. Here we found that SCs in diabetic microenvironment appeared cellular senescence phenotype with excessive reactive oxygen species (ROS) and mitochondrial dysfunction. To target at senescent SCs, we co-entrapped small interfering RNA that targets P53 (Si-P53) and metformin (MET) in ZIF-8-based nanoparticles (MMZ@Si-P53). Mechanistically, MMZ@Si-P53 affected senescent SCs by promoting mitochondria recovery and serine biosynthesis. In addition, rejuvenation of SCs and recovery of mesenchymal stem cell-schwann cell circuitry inhibited bone marrow mesenchymal stem cells (BMSCs) senescence and improved BMSCs ossification. Subsequently MMZ@Si-P53 loaded into glucose-responsive hydrogel system (MMZ@Si-P53/HAMA-PBA-PVA) for triggering release of nanoparticles in hyperglycemia. MMZ@Si-P53/HAMA-PBA-PVA strongly promoted diabetic bone regeneration in diabetic rat model. Our findings demonstrated the effectiveness of MMZ@Si-P53/HAMA-PBA-PVA system in repairing diabetic bone defects.
More Related Videos
Related Concept Videos
Mesenchymal Stem Cells
iPS Cell Differentiation
Tissue Renewal without Stem Cells
However, failure of such a system...
Stem Cell Culture
Stem Cell Niche
Neurogenesis and Regeneration of Nervous Tissue

