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Updated: Jan 18, 2026
Author Spotlight: Advancing Caenorhabditis elegans Research Using Paraformaldehyde-Treated Bacteria
Published on: July 28, 2023
Lactic acid bacteria alleviate neuronal excitotoxicity and extend Caenorhabditis elegans lifespan
Bao LE1, Thi Nhat Hong Nguyen1, Seung-Hwan Yang2
1Research Group in Pharmaceutical and Biomedical Sciences, Faculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City, Vietnam.
Aim:
Neuronal cell death plays a critical role in the development of neurological disorders associated with aging. This study aimed to evaluate the beneficial effects of heat-ki lled lactic acid bacteria (hkLAB) on neuroblastoma cells and Caenorhabditis elegans.
Materials And Methods:
We pretreated heat-killed Lactobacillus fermentum CNU384 (hkCNU384), L. brevis CNU386 (hkCNU386), L. helveticus CNU395 (hkCNU395), and L. paracasei CNU396 (hkCNU396) at a concentration of 109 CFU/mL to investigate their neuroprotective effects on glutamate-induced SH-SY5Y cells and their impact on the lifespan of 6-hydroxydopamine (6-OHDA)-induced C. elegans.
Results:
Our findings indicate that hkCNU395 and hkCNU396 protected against glutamate-induced cell death via modulation of the Bax/Bcl-2 apoptosis regulator ratio and enhancement of endogenous antioxidant enzyme activity. Moreover, hkCNU396 significantly improves survival rates in C. elegans with neuron degeneration induced by 6-OHDA.
Conclusion:
These results highlight hkCNU396 as a promising paraprobiotic candidate for patients suffering from degenerative neurodegenerative diseases.
