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Related Concept Videos

Applications Of NMR In Biology01:25

Applications Of NMR In Biology

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Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
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RNA Structure01:23

RNA Structure

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The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
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RNA Structure01:19

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The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
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RNA Stability01:53

RNA Stability

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Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Updated: Jan 18, 2026

Practical Aspects of Sample Preparation and Setup of 1H R1&#961; Relaxation Dispersion Experiments of RNA
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Advances in solid-state NMR methods for studying RNA structures and dynamics.

Jinhan He1, Xiaole Liu1, Shenlin Wang1

  • 1State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.

Magnetic Resonance Letters
|September 8, 2025
PubMed
Summary
This summary is machine-generated.

Solid-state Nuclear Magnetic Resonance (SSNMR) offers a powerful method for studying Ribonucleic acid (RNA) structure and dynamics. This review highlights advancements in 1H-detected SSNMR techniques for RNA research.

Keywords:
DynamicsPulse sequencesRNASolid-state NMRStructure

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Area of Science:

  • Biophysics
  • Structural Biology
  • Biochemistry

Background:

  • Ribonucleic acid (RNA) structure and dynamics are vital for understanding biological functions and designing RNA-targeting drugs.
  • Conventional methods like X-ray crystallography and solution Nuclear Magnetic Resonance (NMR) face challenges with RNA flexibility and degradation.
  • Solid-state NMR (SSNMR) has emerged as a powerful alternative for RNA structure and dynamics characterization.

Purpose of the Study:

  • To provide a comprehensive review of recent progress in 1H-detected SSNMR for RNA structure and dynamics.
  • To discuss established 1H-detected SSNMR methods and sample preparation protocols.
  • To highlight the implementation of rapid data acquisition approaches in RNA SSNMR.

Main Methods:

  • Focus on 1H-detected solid-state Nuclear Magnetic Resonance (SSNMR) techniques.
  • Detailed discussion of sample preparation protocols for RNA.
  • Exploration of rapid data acquisition strategies for enhanced efficiency.

Main Results:

  • 1H-detected SSNMR methods have advanced the elucidation of RNA topology and base pair dynamics in the solid state.
  • Applications include determining RNA structures in the human immunodeficiency virus (HIV) genome and riboswitch RNAs.
  • SSNMR techniques demonstrate suitability for various RNA sizes and sample states.

Conclusions:

  • Solid-state NMR is a valuable and versatile tool for RNA structure and dynamics research.
  • Advancements in 1H-detected SSNMR have significantly expanded its utility in the RNA field.
  • This review consolidates key methods and findings, paving the way for future RNA structural studies.