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Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

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3D-Printed Microfiltration Membranes via Dual-Wavelength Microstereolithography.

Hanieh Bazyar1, Shang-Che Wu2, Irem Gurbuz2

  • 1Transport Phenomena, Chemical Engineering Department, Faculty of Applied Sciences, Delft University of Technology, Van der Maasweg 9, Delft 2629HZ, The Netherlands.

ACS Omega
|September 8, 2025
PubMed
Summary
This summary is machine-generated.

3D printing offers a sustainable method for creating microfiltration membranes with precise control over pore structure. These novel membranes demonstrate comparable performance to commercial options, successfully separating oil from water emulsions.

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Area of Science:

  • Materials Science
  • Chemical Engineering
  • Additive Manufacturing

Background:

  • Traditional membrane manufacturing is resource-intensive and generates waste.
  • 3D printing offers a sustainable alternative with enhanced control over membrane architecture.
  • Developing precise 3D printing techniques for microfiltration membranes is crucial for advanced separation technologies.

Purpose of the Study:

  • To investigate the 3D printing of microfiltration membranes using a novel dual-wavelength microstereolithography method.
  • To characterize the properties and performance of the 3D-printed membranes.
  • To develop predictive models for membrane permeability and understand factors influencing performance.

Main Methods:

  • Utilized dual-wavelength microstereolithography and gradient descent for precise membrane fabrication.
  • Employed polyethylene glycol diacrylate (PEGDA) to print hydrophilic porous membranes with controlled pore sizes.
  • Characterized membranes using SEM, FTIR, contact angle, and surface roughness measurements.
  • Evaluated pure water permeability and oil-in-water emulsion separation performance.
  • Applied 1D tube and numerical modeling to predict and analyze membrane permeability.

Main Results:

  • Successfully fabricated hydrophilic porous membranes with uniform thickness and micrometer-precision cylindrical pores.
  • 3D-printed membranes exhibited pure water permeability comparable to commercial PTFE membranes.
  • Demonstrated effective separation of oil droplets from oil-in-water emulsions.
  • Investigated material properties and pore deformation effects on permeability predictions.

Conclusions:

  • 3D printing provides a sustainable and precise method for manufacturing microfiltration membranes.
  • The developed membranes show promising performance for emulsion separation applications.
  • Insights into permeability prediction and design optimization for 3D-printed membranes were gained.