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Updated: Jan 18, 2026

Analyzing Protein Dynamics Using Hydrogen Exchange Mass Spectrometry
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Using NMR-detected hydrogen-deuterium exchange to quantify protein stability in cosolutes, under crowded conditions

I-Te Chu1, Gary J Pielak1,2,3,4

  • 1Department of Chemistry, University of North Carolina at Chapel Hill (UNC-CH), Chapel Hill, NC, 27599-3290, United States.

Magnetic Resonance Letters
|September 8, 2025
PubMed

Keywords:
Amide proton exchangeCosolutesCrowdingEquilibrium thermodynamicsMacromolecularOsmolytesProtein stability

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