Related Experiment Video
Updated: Jan 18, 2026

NMR Spectroscopy as a Robust Tool for the Rapid Evaluation of the Lipid Profile of Fish Oil Supplements
Published on: May 1, 2017
1H and 13C NMR spectral assignments for low-concentration bile acids in biological samples
Hong Lin1, Junbo He1, Weinong Zhang1
1MOE Key Laboratory for Deep Processing of Major Grain and Oil, School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China.
Abstract:
Bile acids are the main body of enterohepatic circulation in vivo. They have essential functions such as emulsifying fat, bacteriostasis and regulating multiple metabolic pathways as signal molecules. However, the assignments of NMR signals for some low-concentration bile acids are still needed. This study combined 1D nuclear magnetic resonance (NMR) and 2D NMR techniques including 1H-1H correlation spectroscopy (COSY), 1H-1H total correlation spectroscopy (TOCSY), 1H J-resolved spectroscopy (J-Res), 1H-13C heteronuclear single quantum coherence spectroscopy (HSQC), and 1H-13C heteronuclear multiple bond correlation spectroscopy (HMBC) to assign the 1H and 13C signals of six bile acids in aqueous solution at physiological pH (∼7.4) and nine bile acids in methanol. These data are of importance to the NMR-based studies on lipid digestion, absorption, and metabolism.
More Related Videos
Related Concept Videos
NMR and Mass Spectroscopy of Carboxylic Acids
While α protons of carboxylic acids absorb at 2–2.5 ppm, β protons absorb further upfield.
Carboxylic acids are easily identified by dissolving them in deuterium oxide, which results in a rapid exchange of the acidic protons with deuterium. This leads to the...
Carbon-13 (¹³C) NMR: Overview
Proton (¹H) NMR: Chemical Shift
Absorption signals of all the protium nuclei...
¹³C NMR: ¹H–¹³C Decoupling
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
Spectroscopy of Carboxylic Acid Derivatives
NMR Spectroscopy and Mass Spectrometry of Aldehydes and Ketones

