Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

18.5K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
18.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sturgeon collagen fibrils as a promising biomaterial for cartilage tissue engineering.

Journal of biomaterials applications·2026
Same author

FRET-SAM: SAM_Med2D-based automatic FRET two-hybrid analysis.

Computer methods and programs in biomedicine·2025
Same author

A theoretical study on the mechanism of C<sub>2</sub>H<sub>3-5</sub> oxidation by N<sub>2</sub>O.

Physical chemistry chemical physics : PCCP·2025
Same author

High-accuracy theoretical studies on the gas-phase reaction mechanisms of sulfur mustard with reactive oxygen species (OH/O<sub>2</sub>/HO<sub>2</sub>/O).

Physical chemistry chemical physics : PCCP·2025
Same author

Development of dual base editors based on ssDNA-targeting SCP1.201 deaminases for the artificial evolution of novel herbicide-tolerant OsEPSPS variants.

Plant communications·2025
Same author

Transcriptome-wide identification and expression analysis of lncRNAs involved in the nitrate response in wheat.

BMC plant biology·2025

Related Experiment Video

Updated: Jan 18, 2026

Magnetic-, Acoustic-, and Optical-Triple-Responsive Microbubbles for Magnetic Hyperthermia and Pothotothermal Combination Cancer Therapy
09:01

Magnetic-, Acoustic-, and Optical-Triple-Responsive Microbubbles for Magnetic Hyperthermia and Pothotothermal Combination Cancer Therapy

Published on: May 22, 2020

3.5K

Calcium/Manganese Nanoreactors Enable Triple-Enhanced Chemodynamic/Photodynamic Therapy via Tumor Microenvironment

Huanhuan Wang1,2,3, Ting Zhang1,2, Yan Zhuang1,2

  • 1MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, School of Optoelectronic Science and Engineering, South China Normal University, No.55 West Zhongshan Avenue, Tianhe District, Guangzhou 510631, Guangdong, China.

ACS Applied Materials & Interfaces
|September 8, 2025
PubMed
Summary

This study developed a novel nanoreactor that overcomes tumor microenvironment barriers to enhance cancer treatment. The nanoreactor effectively reprograms the tumor environment, boosting chemodynamic and photodynamic therapies for improved efficacy.

Keywords:
calcium/manganese nanoreactorchemodynamic therapyphotodynamic therapyreactive oxygen species stormtumor microenvironment reprogramming

More Related Videos

An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model
11:04

An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model

Published on: January 13, 2023

3.6K
Anticancer Efficacy of Photodynamic Therapy with Lung Cancer-Targeted Nanoparticles
08:03

Anticancer Efficacy of Photodynamic Therapy with Lung Cancer-Targeted Nanoparticles

Published on: December 1, 2016

9.5K

Related Experiment Videos

Last Updated: Jan 18, 2026

Magnetic-, Acoustic-, and Optical-Triple-Responsive Microbubbles for Magnetic Hyperthermia and Pothotothermal Combination Cancer Therapy
09:01

Magnetic-, Acoustic-, and Optical-Triple-Responsive Microbubbles for Magnetic Hyperthermia and Pothotothermal Combination Cancer Therapy

Published on: May 22, 2020

3.5K
An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model
11:04

An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model

Published on: January 13, 2023

3.6K
Anticancer Efficacy of Photodynamic Therapy with Lung Cancer-Targeted Nanoparticles
08:03

Anticancer Efficacy of Photodynamic Therapy with Lung Cancer-Targeted Nanoparticles

Published on: December 1, 2016

9.5K

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Cancer Therapy

Background:

  • Reactive oxygen species (ROS)-based therapies like chemodynamic therapy (CDT) and photodynamic therapy (PDT) show promise for cancer treatment.
  • Tumor microenvironment (TME) factors such as glutathione (GSH) overexpression, low hydrogen peroxide (H2O2) levels, and hypoxia limit the efficacy of CDT and PDT.

Purpose of the Study:

  • To engineer a nanoreactor that overcomes TME barriers to enhance synergistic CDT and PDT.
  • To develop a Trojan horse-inspired nanoreactor for sequential TME reprogramming and cascading ROS generation.

Main Methods:

  • Fabrication of a MnO2-shelled CaO2 nanoreactor (CaO2/MnO2-Ce6-PEG) with a sequential TME reprogramming strategy.
  • In vitro evaluation of nanoreactor cellular uptake, GSH depletion, H2O2/O2 generation, and synergistic CDT/PDT effects.
  • In vivo assessment of tumor growth inhibition and systemic toxicity in murine models.

Main Results:

  • The nanoreactor successfully depleted intracellular GSH, generated H2O2 and O2, and exhibited synergistic CDT and PDT effects in cancer cells.
  • In vivo studies demonstrated potent tumor growth inhibition with minimal systemic toxicity.
  • The nanoreactor's Trojan horse architecture facilitated TME-responsive shell dissociation and controlled payload release.

Conclusions:

  • The designed nanoreactor effectively reprograms the TME by blocking GSH, replenishing ROS precursors, and reversing hypoxia, leading to triple-enhanced CDT/PDT.
  • This self-reinforcing nanoplatform offers a promising strategy for overcoming TME barriers in ROS-based cancer therapy.