Multi drug resistant Pseudomonas aeruginosa in burn infection among Iraq patients

Area of Science:

• Microbiology
• Molecular Biology
• Infectious Diseases

Background:

• Pseudomonas aeruginosa is a significant opportunistic pathogen in burn wound infections.
• Multidrug resistance (MDR) in P. aeruginosa contributes to high morbidity and mortality.
• Understanding the resistance profile and genetic relatedness is crucial for effective treatment.

Purpose of the Study:

• To evaluate the multidrug resistance profile of P. aeruginosa isolates from human burn infections.
• To perform molecular phylogenetic analysis of these isolates.
• To identify specific antibiotic resistance patterns and genetic relatedness.

Main Methods:

• Isolation and identification of P. aeruginosa using traditional culture and biochemical tests.
• Antibiotic susceptibility testing via disc diffusion method (CLSI guidelines).
• Molecular identification and phylogenetic analysis using PCR and Sanger sequencing of the 16S rRNA gene.

Main Results:

• Isolates exhibited multidrug-resistant phenotypes, with notable resistance to cefixime and meropenem.
• PCR confirmed P. aeruginosa presence via 16S rRNA gene amplification.
• Phylogenetic analysis showed high genetic similarity to global P. aeruginosa strains.

Conclusions:

• The P. aeruginosa isolates from burn wounds possess significant multidrug resistance.
• Genetic analysis indicates potential international dissemination or conserved evolutionary traits.
• Findings highlight the need for monitoring MDR P. aeruginosa in clinical settings.