Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Conjugated Proteins02:50

Conjugated Proteins

27.0K
Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
27.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluating immunoreactivity of hybridoma-derived antibody mixtures generated against AU-565 cell line for diagnosis and immunotherapy of breast cancer.

BMC cancer·2026
Same author

Adjuvant choice shapes macrophage activation and cytokine responses to freeze-thaw breast cancer antigens.

BMC research notes·2026
Same author

Poly(ε-caprolactone) Nanoparticle Tumor-Lysate Vaccination in Mice Generates Hybridoma-Derived Antibodies Enabling Breast Cancer Diagnosis and Chemotherapy Synergy.

Biomedicines·2026
Same author

Tri-Layer Composite Nanofiber Wound Dressing Incorporating Glucantime and Silver Nanoparticles for Cutaneous Leishmaniasis Management.

Journal of functional biomaterials·2026
Same author

Deglycosylation of VEGF Produced in Pichia pastoris Reduces its in Vitro Binding and Angiogenic Effect.

Biotechnology journal·2025
Same author

Engineering Two-in-One Nanoparticles for Simultaneous Delivery of Graphene Quantum Dot and Pemetrexed.

ACS omega·2025

Related Experiment Video

Updated: Jan 6, 2026

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain
08:07

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

Published on: July 25, 2022

3.0K

Development of Receptor-Binding Domain (RBD)-Loaded PEG-PCL Nanoparticle Formulations Against SARS-CoV-2.

Sena Ayçiçek Can1,2, Selin Göksever1,2, Umut Can Öz1

  • 1Department of Pharmaceutical Technology, Faculty of Pharmacy, Ankara University, Tandogan, Ankara, Turkey.

Macromolecular Bioscience
|September 8, 2025
PubMed
Summary

This study developed novel nanovaccines using SARS-CoV-2 receptor-binding domain (RBD) encapsulated in PEG-PCL nanoparticles. These formulations show promise for stable antigen delivery and cellular activation, supporting future COVID-19 vaccine development.

Keywords:
COVID‐19SARS‐CoV‐2polymeric nanoparticlesreceptor‐binding domainsubunit vaccine

More Related Videos

Engineering Antiviral Agents via Surface Plasmon Resonance
13:00

Engineering Antiviral Agents via Surface Plasmon Resonance

Published on: June 14, 2022

2.7K
Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques
08:07

Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques

Published on: January 12, 2024

1.2K

Related Experiment Videos

Last Updated: Jan 6, 2026

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain
08:07

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

Published on: July 25, 2022

3.0K
Engineering Antiviral Agents via Surface Plasmon Resonance
13:00

Engineering Antiviral Agents via Surface Plasmon Resonance

Published on: June 14, 2022

2.7K
Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques
08:07

Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques

Published on: January 12, 2024

1.2K

Area of Science:

  • Nanotechnology
  • Vaccine Development
  • Immunology

Background:

  • The COVID-19 pandemic necessitates the development of safe and effective vaccines.
  • Subunit nanovaccines offer a promising approach for targeted antigen delivery.

Purpose of the Study:

  • To formulate and characterize poly(ethylene glycol)-block-poly(ε-caprolactone) (PEG-PCL) nanoparticles for delivering the SARS-CoV-2 receptor-binding domain (RBD).
  • To evaluate the impact of different surfactants (PVA and SC) on nanovaccine properties and performance.

Main Methods:

  • Utilized a modified water-in-oil-in-water (w1/o/w2) emulsion-solvent evaporation method for nanoparticle formulation.
  • Characterized nanoparticles for size, surface charge, encapsulation efficiency, and morphology.
  • Assessed antigen integrity, in vitro release kinetics, and cellular activation via nitric oxide (NO) production.

Main Results:

  • Optimized PEG-PCL nanoparticles achieved sizes below 300 nm with high encapsulation efficiency (>80%).
  • Nanoparticles demonstrated sustained release of the RBD antigen over time.
  • Both PVA- and SC-containing formulations induced comparable levels of cellular activation in dendritic cells.

Conclusions:

  • PEG-PCL nanovaccine systems provide a stable and effective platform for delivering viral antigens like SARS-CoV-2 RBD.
  • These nanovaccines represent a promising strategy for developing next-generation vaccines against COVID-19 and similar viral threats.