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Updated: Jan 18, 2026

High-Throughput Capable Three-Dimensional Tissue Model for Quantification of Electroporation Thresholds
08:23

High-Throughput Capable Three-Dimensional Tissue Model for Quantification of Electroporation Thresholds

Published on: August 19, 2025

551

High-Throughput Capable Three-Dimensional Tissue Model for Quantification of Electroporation Thresholds.

Robert H Williamson1, Alexia K Cash2, Anna E Riordan2

  • 1North Carolina State University; rhwilli4@ncsu.edu.

Journal of Visualized Experiments : Jove
|September 8, 2025
PubMed
Summary

This study introduces a high-throughput 3D tissue model to efficiently determine electroporation thresholds. This method accelerates the characterization of electroporation protocols for applications like gene therapy and cancer treatment.

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Medical Engineering

Background:

  • Electroporation uses electrical pulses for macromolecule delivery and tissue ablation.
  • Current methods for determining electroporation thresholds are time-intensive and not fully representative of in vivo conditions.

Purpose of the Study:

  • To develop and validate a high-throughput 3D tissue culture model for quantifying electroporation thresholds.
  • To enhance the efficiency of characterizing electroporation protocols for in vivo applications.

Main Methods:

  • Utilized a non-uniform electric field in a 3D tissue mimic with HEK293 cells.
  • Employed a ring and pin electrode system to deliver a GFP-encoding plasmid.
  • Analyzed spatial distribution of transfected cells via fluorescent microscopy to derive thresholds.

Main Results:

  • Successfully identified both reversible and irreversible electroporation thresholds within a single sample.
  • Demonstrated the model's capability to efficiently characterize electroporation protocols.
  • Achieved higher throughput compared to existing threshold evaluation methods.

Conclusions:

  • The developed 3D tissue model offers a high-throughput and more in vivo-representative approach for evaluating electroporation protocols.
  • This method can accelerate the optimization of electroporation for therapeutic applications, including genetic disease treatment and cancer therapy.