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Raman scattering from nucleic acids adsorbed at a silver electrode.

V Brabec, K Niki

    Biophysical Chemistry
    |November 1, 1985
    PubMed
    Summary

    Surface-enhanced Raman scattering (SERS) reveals that single-stranded nucleic acids adsorb to silver electrodes, showing distinct spectral signals. Double-helical nucleic acids, however, do not exhibit these signals, indicating structural stability upon adsorption.

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    Area of Science:

    • Electrochemistry
    • Spectroscopy
    • Biochemistry

    Background:

    • Nucleic acid adsorption at electrode surfaces is crucial for electrochemical biosensors.
    • Understanding nucleic acid structure and stability during adsorption is key for developing reliable biosensing platforms.

    Purpose of the Study:

    • To investigate the adsorption behavior of nucleic acids on a silver electrode using surface-enhanced Raman scattering (SERS).
    • To differentiate between single-stranded and double-helical nucleic acid structures based on their SERS spectra upon adsorption.
    • To assess the structural integrity of nucleic acids after adsorption at the electrode surface.

    Main Methods:

    • Electrochemical polarization of a silver electrode.
    • Surface-enhanced Raman scattering (SERS) spectroscopy.

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  • Analysis of SERS spectra for characteristic adenine residue vibrations.

    • Main Results:

    • Single-stranded polyriboadenylic acid and denatured DNA showed intense SERS bands at 734 and 1335 cm-1, attributed to adenine vibrations.
    • Double-helical nucleic acids (polyadenylic X polyuridylic acid and native DNA) exhibited significantly less intense bands.
    • SERS spectroscopy successfully monitored alkaline denaturation and enzymatic digestion of DNA, correlating with spectral changes.

    Conclusions:

    • Adsorption of nucleic acids onto a silver electrode surface is dependent on their secondary structure.
    • Intact double-helical segments of nucleic acids remain stable and are not denatured or destabilized upon adsorption.
    • SERS spectroscopy is a viable method for probing nucleic acid structure and adsorption dynamics at electrode interfaces.