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Updated: Jan 18, 2026

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model
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Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model

Published on: March 20, 2020

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lncRNA-miRNA/RBP-mRNA Network Involved in Human Triple-Negative Breast Cancer: An Integrated Approach

Wen Yun1, Yao Li2, Jianyuan Huang3

  • 1Department of General Surgery, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing 210009, China.

Journal of Environmental Pathology, Toxicology and Oncology : Official Organ of the International Society for Environmental Toxicology and Cancer
|September 8, 2025
PubMed

Insights

This study reveals a novel RNA regulatory network in triple-negative breast cancer (TNBC). The TMEM161B-AS1-miR-3646-SERPINB5 axis impacts TNBC cell functions, offering new therapeutic targets.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Noncoding RNAs are critical in human breast cancer.
  • Triple-negative breast cancer (TNBC) requires further understanding of its molecular mechanisms.

Purpose of the Study:

  • To construct a comprehensive regulatory network involving multi-type RNAs and RNA-binding proteins (RBPs) in TNBC.
  • To identify key molecular players and their interactions within this network.

Main Methods:

  • Differential expression analysis of lncRNAs, miRNAs, and genes using GEO2R.
  • Identification of downstream RBPs and miRNAs via databases.
  • Network construction including lncRNA-miRNA-mRNA and lncRNA-RBP-mRNA interactions.
  • Validation using TCGA, ENCORI, real-time PCR, and RIP assays.

Main Results:

  • A novel TMEM161B-AS1-miR-3646-SERPINB5 axis was identified, affecting TNBC cell proliferation, migration, and invasion.
  • TMEM161B-AS1 demonstrated inhibitory effects, miR-3646 reversed these effects, and SERPINB5 counteracted miR-3646.
  • RBFOX1 was found to bind SERPINB5 mRNAs, with TMEM161B-AS1 influencing this interaction.

Conclusions:

  • The study established a multi-type RNA and RBP network in TNBC.
  • The identified TMEM161B-AS1-miR-3646-SERPINB5 axis and TMEM161B-AS1-RBFOX1-SERPINB5 axis provide insights into TNBC pathogenesis.
  • These findings offer potential directions for future TNBC research and therapeutic strategies.

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