The Function of Hsa_circ_0001862 Regulating miR-23a-IRF1/PPP2R5E Axis to Oral Squamous Cell Carcinoma Development

Head and neck cancer is among the top ten cancers worldwide, with most lesions in the oral cavity. Oral squamous cell carcinoma accounts for more than 90% of all oral malignancies and is a significant public health concern. Circular RNA and micro RNA, as non-coding RNA, plays an important role in the development of tumor transmission.

This study attempted to explore the pathogenesis of oral squamous cell carcinoma from the perspective of non-coding RNA.

We found that miR-23a was abnormally elevated in oral squamous cell carcinoma tissues. Taking this as an entry point, we searched for the circRNA and downstream target genes of miR-23a's upstream function through bioinformatics prediction. We determined the direct targeting relationship between miR-23a and hsa_circ_0001862, as well as the downstream target genes IRF1 and PPP2R5E, by dual luciferase reporter experiments. We used a series of cell phenotype experiments, such as CCK8, colony formation, scratch and trans-well assays, to verify the influence of each of the above genes on the tongue squamous cell line Tca-8113.

Hsa_circ_0001862 regulates the expression level of miR-23a through sponging. In the occurrence and development of tongue squamous cell carcinoma, the expression level of miR-23a is abnormally increased, which can promote the occurrence and development of tongue squamous cell carcinoma by down-regulating the expression level of its target genes IRF1 and PPP2R5E.

The identification of this signaling pathway provides a practical basis for early detection, early diagnosis and early treatment of oral squamous cell carcinoma.

The regulatory mechanism of hsa_circ_0001862-miR-23a-IRF1/PPP2R5E axis on the OSCC and the function of downstream target genes that play specific roles.