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Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study
Published on: August 16, 2019
Green Synthetic Amide-Modified Hyper-Cross-Linked Adsorption Resin for Efficiently Separating Chlorogenic Acid From
Xuefang Chen1,2,3, Zhijie Shen1,2, Haifeng Chen3
1Guangzhou Institute of Energy Conversion, CAS Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou, People's Republic of China.
Abstract:
Eucommia ulmoides Oliver leaf is rich in chlorogenic acid, which has antioxidant, antiviral, and anti-inflammatory activities. In this work, a new and green strategy for functional hyper-crosslinked adsorption resin based on Friedel-Crafts reaction of pendant vinyl groups in divinylbenzene with anhydrous ethanol and acrylamide grafting polymerization was developed, and the obtained HCREt-AM resin had excellent performance on chlorogenic acid separation from Eucommia ulmoides Oliver leaf extract. Adsorption isotherm and kinetics study showed the adsorption process fitted by Langmuir adsorption isotherm and pseudo-second-order kinetic equation. The dynamic saturated adsorption capacity of 88.0 mg·g-1 was obtained, and chlorogenic acid content was raised from 7.91% to 48.27%. The pH-controlled ionization of chlorogenic acid determined the adsorption process, and π-π stacking, hydrophobic interactions, hydrogen bonding, and electrostatic interactions are the main adsorption mechanisms.
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