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Related Concept Videos

Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

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Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
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Super-Resolution Compatible DNA Labeling Technique Reveals Chromatin Mobility and Organization Changes During

Maruthi K Pabba1, Miroslav Kuba2,3, Tomáš Kraus2

  • 1Cell Biology and Epigenetics, Department of Biology, Technical University of Darmstadt, 64287, Darmstadt, Germany.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|September 9, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed a new probe for tracking chromatin mobility in living cells during neural differentiation. This method reveals that chromatin becomes less mobile and accessible as cells differentiate, offering insights into cell fate commitment.

Keywords:
SNTT1STEDchromatin mobilitydCSiRTPhuman iPSC differentiation

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Area of Science:

  • Cell Biology
  • Neuroscience
  • Genetics

Background:

  • Chromatin dynamics are vital for cellular differentiation, but studying global chromatin mobility in live cells is challenging.
  • Existing tools for observing chromatin behavior during differentiation are limited.

Purpose of the Study:

  • To develop and validate a novel metabolic labeling probe for tracking chromatin dynamics in living cells.
  • To investigate changes in chromatin mobility and organization during neural differentiation.

Main Methods:

  • Metabolic labeling of chromatin using a novel silicon rhodamine-conjugated deoxycytidine triphosphate (dCSiRTP) delivered by a synthetic transporter (SNTT1).
  • Correlative confocal and STED super-resolution microscopy for quantifying chromatin domain sizes.
  • Time-lapse super-resolution microscopy with single particle tracking to analyze chromatin mobility.
  • Micrococcal nuclease digestion assays, chromatin compaction, and histone modification analyses.

Main Results:

  • The dCSiRTP probe was successfully delivered into human induced pluripotent stem cells (iPSCs) and neural stem cells (NSCs).
  • Chromatin mobility significantly decreased during the transition from iPSCs to neurons.
  • Neuronal differentiation correlated with reduced chromatin accessibility and increased heterochromatin-associated histone modifications.

Conclusions:

  • The developed probe enables real-time tracking of chromatin dynamics in living cells during differentiation.
  • Chromatin undergoes structural changes, becoming more compact and less accessible during neurogenesis.
  • These findings provide novel insights into the regulation of chromatin organization during cell fate commitment.