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Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Updated: Jan 18, 2026

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

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Published on: February 9, 2024

Bioinspired DNA Framework-Programmed Heteroligation for Affinity-Enhanced Biodetection.

Shuai Yang¹, Chenghao Xi², Xiaolei Zuo²

¹Central Laboratory, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.

|September 9, 2025

Summary

This summary is machine-generated.

Researchers engineered a DNA framework to precisely control multivalent interactions for enhanced molecular detection. This DNA framework enables ultrasensitive detection of platelet-derived growth factor-BB (PDGF-BB), with potential applications in diagnostics and immunotherapy.

Keywords:

DNA origami aptamers electrochemical sensing heteroligation

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Published on: September 10, 2014

Area of Science:

Biochemistry
Molecular Biology
Nanotechnology

Background:

Natural polyreactive antibodies utilize heterogeneous ligand binding for enhanced avidity.
Engineering synthetic heteroligation systems with precise control over recognition motif orientation and distance is challenging.

Purpose of the Study:

To present a DNA framework-based strategy for programming heterotypic binding.
To demonstrate programmable modulation of binding affinity by tuning the distance between heterotypic recognition motifs.
To achieve ultrasensitive detection of platelet-derived growth factor-BB (PDGF-BB).

Main Methods:

Utilizing a DNA framework to spatially organize bivalent aptamers targeting PDGF-BB.
Systematically tuning the distance between heterotypic recognition motifs on the DNA framework.
Characterizing the binding kinetics, specificity, and sensitivity of the engineered heteroligation system.

Main Results:

Demonstrated programmable modulation of binding affinity by adjusting motif distance.
Achieved rapid binding kinetics and high specificity for PDGF-BB detection.
Reached an ultrasensitive limit of detection for PDGF-BB at 0.5 pM.

Conclusions:

The DNA framework provides a versatile platform for designing and controlling multivalent interactions.
This approach offers potential applications in disease diagnostics and immunotherapy for various disorders.
Precise spatial organization of aptamers on DNA frameworks enables enhanced molecular recognition and detection.