Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Development of indicators to evaluate the effectiveness of antimicrobial stewardship programs in Korea: a Delphi consensus study.

Antimicrobial resistance and infection control·2026

Same author

Performance and Cost-Efficiency of an MPT64 Assay for Detecting <i>Mycobacterium tuberculosis</i> Complex in a Setting With Frequent Nontuberculous Mycobacteria Isolation.

Journal of Korean medical science·2026

Same author

Ensitrelvir for the treatment of hospitalized adults with COVID-19: an international phase 3 randomized placebo-controlled trial.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America·2026

Same author

Risk Factors for Immune Recovery Uveitis in Patients with Cytomegalovirus Retinitis After Hematopoietic Stem Cell Transplantation.

Ocular immunology and inflammation·2026

Same author

Real-World Effectiveness of a Disinfectant Wipe on Healthcare Surfaces: A Multi-Center Study in Five University-Affiliated Hospitals.

Infection & chemotherapy·2026

Same author

Pharmacokinetic Model Based on Stochastic Simulation and Estimation for Therapeutic Drug Monitoring of Teicoplanin in Korean Neutropenic Hematopoietic Stem Cell Transplant Recipients.

Drug design, development and therapy·2026

Search research articles

Related Experiment Video

Updated: Jan 18, 2026

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis

Published on: March 9, 2018

Evaluation of a Manual DNA Extraction Method Combined with In-House Aspergillus Real-Time PCR.

In Young Yoo, Joo An Kwon, Raeseok Lee

Clinical Laboratory

|September 9, 2025

Summary

A novel DNA extraction method using beads and resin improves Aspergillus detection in respiratory samples. This method shows higher sensitivity and lower cycle threshold values than a commercial kit, aiding in diagnosing invasive pulmonary aspergillosis.

More Related Videos

Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA

Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA

Published on: May 2, 2018

A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples

A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples

Published on: December 10, 2014

Related Experiment Videos

Last Updated: Jan 18, 2026

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis

Published on: March 9, 2018

Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA

Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA

Published on: May 2, 2018

A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples

A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples

Published on: December 10, 2014

Area of Science:

Molecular Diagnostics
Mycology
Clinical Microbiology

Background:

Improving molecular diagnostic yield for Aspergillus species from respiratory samples is crucial.
Development of novel DNA extraction techniques is key for enhanced pathogen detection.

Purpose of the Study:

To develop and evaluate a new DNA extraction method for Aspergillus spp. directly from respiratory samples.
To compare the efficiency of the new method with a commercial kit (Qiagen) using in-house Aspergillus real-time PCR.

Main Methods:

A beads and resin-based DNA extraction method was developed, involving bead beating, supernatant mixing, washing, and Chelex-100 resin treatment.
Nucleic acids were extracted from contrived sputum samples and 100 clinical respiratory specimens using both the new method and the Qiagen kit.
Extracted DNA was analyzed using in-house Aspergillus real-time PCR, with cycle threshold (Ct) values compared between methods.

Main Results:

The beads plus resin method yielded significantly lower Ct values for Aspergillus niger and Aspergillus flavus in contrived samples compared to the Qiagen kit.
In clinical samples, the beads plus resin method achieved a higher sensitivity (88%) than the Qiagen kit (76%) for pan-Aspergillus detection.
The new method consistently produced significantly lower Ct values in clinical specimens (p < 0.001).

Conclusions:

A novel DNA extraction method using beads and resin effectively detects Aspergillus spp. directly from respiratory specimens.
This method outperforms a commercial kit, offering improved sensitivity and efficiency for diagnosing invasive pulmonary aspergillosis.