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Protein Denaturation01:28

Protein Denaturation

The function of proteins depends on their native three-dimensional structure, which is dictated by the amino acid sequence of the specific protein. Folding of the polypeptide chain takes place under specific conditions that energetically favor the folded conformation. In contrast, protein denaturation occurs spontaneously under unfavorable conditions that disrupt the integrity of the folded conformation. Thus, the chemical and physical environment of a protein, such as significant changes in pH...

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Diagnostic Necropsy and Selected Tissue and Sample Collection in Rats and Mice
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Time-dependent post-mortem histomorphological changes in Penaeus vannamei following humane euthanasia.

Rodolfo Lozano-Olvera1, Karla G Aguilar-Rendón2, Sergio Cervantes3

  • 1Centro de Investigación en Alimentación y Desarrollo, A.C. Subsede Mazatlán en Acuicultura y Manejo Ambiental, Av. Sábalo-Cerritos S/N, Mazatlán 82112, Sinaloa, México. rlozano@ciad.mx.

Veterinary Research Communications
|September 9, 2025
PubMed
Summary

Post-mortem autolysis in white shrimp (Penaeus vannamei) occurs rapidly, significantly impacting histological analysis. Immediate sample fixation within 5 minutes of death is crucial to prevent misinterpretation of tissue changes.

Keywords:
P. vannameiRigor mortisAutolysisHepatopancreasPost-mortem time

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Area of Science:

  • Aquatic animal health
  • Histopathology
  • Post-mortem analysis

Background:

  • Histological analysis is vital for evaluating animal tissues and abnormalities.
  • Post-mortem autolysis can alter tissue structure, complicating histopathological interpretation.
  • Understanding autolysis progression in shrimp is essential for accurate diagnostic sampling.

Purpose of the Study:

  • To analyze post-mortem histomorphological changes in white shrimp (Penaeus vannamei).
  • To determine the optimal time for collecting histopathological samples after euthanasia.
  • To evaluate autolysis progression in key shrimp tissues.

Main Methods:

  • Euthanasia of 80 juvenile white shrimp using clove oil and pithing.
  • Post-mortem analysis at set intervals (0-360 minutes).
  • Examination of color, rigor mortis, and tissue structure (hepatopancreas, midgut, antennal gland).

Main Results:

  • Rapid autolysis observed, particularly in the hepatopancreas.
  • Tissue integrity loss noted as early as 10 minutes post-mortem.
  • Significant correlation between post-mortem time and autolysis degree (r > 0.90).

Conclusions:

  • Post-mortem changes in Penaeus vannamei are rapid and affect histopathological accuracy.
  • Hepatopancreas shows the fastest autolysis rate, followed by midgut and antennal gland.
  • Immediate sample fixation (within 5 minutes) is recommended to avoid misinterpretation.