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Updated: Jan 18, 2026
Generation of Genome-wide Chromatin Conformation Capture Libraries from Tightly Staged Early Drosophila Embryos
Published on: October 3, 2018
Small-scale in situ Hi-C protocol for early embryos to resolve the three-dimensional genome structure
Linjie Song1, Jingcheng Zhang2, Linqian Pan1
1College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China; Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling 712100, China.
Abstract:
High-throughput chromosome conformation capture (Hi-C) provides genome-wide insights into chromatin interactions within the three-dimensional structure of the nucleus, making it a powerful tool for studying genome architecture. Here, we provide a modified in situ Hi-C protocol for small cell numbers, utilizing 50-100 embryonic cells at the 8-cell stage to investigate chromatin organization during bovine early embryonic development. This protocol overcomes the challenges of limited sample availability and offers valuable insights into chromatin dynamics during bovine early embryogenesis. For complete details on the use and execution of this protocol, please refer to Zhang et al.1.
